验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MDA-MB-231 cells, OVCAR-3 cells, SKOV-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30010-1-AP targets ITGBL1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag31989 Product name: Recombinant human ITGBL1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 246-374 aa of BC036788 Sequence: VCGECTCHDVDPTGDWGDIHGDTCECDERDCRAVYDRYSDDFCSGHGQCNCGRCDCKAGWYGKKCEHPQSCTLSAEESIRKCQGSSDLPCSGRGKCECGKCTCYPPGDRRVYGKTCECDDRRCEDLDGV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | integrin, beta-like 1 (with EGF-like repeat domains) |
| 别名 | Integrin beta like protein 1, OSCP, Osteoblast-specific cysteine-rich protein, Ten integrin EGF-like repeat domain-containing protein, TIED |
| 计算分子量 | 494 aa, 54 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC036788 |
| 基因名称 | ITGBL1 |
| Gene ID (NCBI) | 9358 |
| RRID | AB_3086208 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95965 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Integrin beta-like protein 1 (ITGBL1), also named as TIED or OSCP, is a secreted protein that contains ten tandem EGF-like repeats strikingly similar to those found in the cysteine-rich "stalk-like" structure of integrin beta subunits (PMID: 10051402). ITGBL1 participates in the regulation of fibrogenesis via interactions with transforming growth factor beta 1 (PMID: 28262670). ITGBL1 is also involved in the development and metastasis of many tumors (PMID: 32211321; 29772438; 26060017; 31190876).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ITGBL1 antibody 30010-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
