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验证数据展示

  • WB analysis of HL-60 using 60258-1-Ig

    WB analysis of HL-60 using 60258-1-Ig

    Untreated and PNGase F-treated lysates of HL-60 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

  • WB analysis of human spleen using 60258-1-Ig

    WB analysis of human spleen using 60258-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 60258-1-Ig

    IHC staining of human tonsillitis using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60258-1-Ig (CD11c/Integrin Alpha X antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD11c antibody included
  • IHC staining of human tonsillitis using 60258-1-Ig

    IHC staining of human tonsillitis using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60258-1-Ig (CD11c/Integrin Alpha X antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD11c antibody included
  • IF Staining of human tonsillitis using 60258-1-Ig

    IF Staining of human tonsillitis using 60258-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11c/Integrin Alpha X antibody (60258-1-Ig, Clone: 2F1C10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human tonsillitis using 60258-1-Ig

    IF Staining of human tonsillitis using 60258-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11c/Integrin Alpha X antibody (60258-1-Ig, Clone: 2F1C10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human appendicitis using 60258-1-Ig

    IHC staining of human appendicitis using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60258-1-Ig (CD11c antibody) at dilution of 1:15000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human ovary cancer using 60258-1-Ig

    IHC staining of human ovary cancer using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 60258-1-Ig (CD11c antibody) at dilution of 1:15000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human liver using 60258-1-Ig

    IHC staining of human liver using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60258-1-Ig (CD11c antibody) at dilution of 1:15000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human tonsillitis using 60258-1-Ig

    IHC staining of human tonsillitis using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60258-1-Ig (CD11c antibody) at dilution of 1:15000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human appendicitis using 60258-1-Ig

    IHC staining of human appendicitis using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60258-1-Ig (CD11c/Integrin Alpha X antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD11c antibody included
  • IHC staining of human liver cancer using 60258-1-Ig

    IHC staining of human liver cancer using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60258-1-Ig (CD11c/Integrin Alpha X antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD11c antibody included
  • IHC staining of human tonsillitis using 60258-1-Ig

    IHC staining of human tonsillitis using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60258-1-Ig (CD11c antibody) at dilution of 1:15000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD11c antibody included
  • IHC staining of human liver using 60258-1-Ig

    IHC staining of human liver using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60258-1-Ig (CD11c antibody) at dilution of 1:15000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD11c antibody included
  • IHC staining of human appendicitis using 60258-1-Ig

    IHC staining of human appendicitis using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60258-1-Ig (CD11c antibody) at dilution of 1:15000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD11c antibody included
  • IHC staining of human ovary cancer using 60258-1-Ig

    IHC staining of human ovary cancer using 60258-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 60258-1-Ig (CD11c antibody) at dilution of 1:15000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD11c antibody included

CD11c Monoclonal antibody

CD11c Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 60258-1-Ig

产品说明书

CloneNo. 2F1C10
引用文献(33)

宿主/亚型

Mouse / IgG2a

种属反应性

human and More (2)

应用

WB, IHC, IF-P, ELISA and More (1)

别名

2F1C10, CD11 antigen-like family member C, Integrin alpha X, Integrin alpha-X, ITGAX

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman spleen tissue, THP-1 cells, HL-60 cells, U-937 cells
Positive IHC detected inhuman tonsillitis tissue, human breast cancer tissue, human tonsillitis tissue, human liver tissue, human ovary cancer tissue, human lung cancer tissue, human appendicitis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue, human appendicitis tissue
Planning an IHC experiment? We recommend our IHCeasy CD11c Ready-To-Use IHC Kit. CD11c primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:10000-1:40000
Immunofluorescence (IF)-PIF-P : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60258-1-Ig targets CD11c in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, rat, pig
免疫原

CatNo: Ag11350

Product name: Recombinant human CD11c/Integrin alpha X protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 21-352 aa of BC038237

Sequence: NLDTEELTAFRVDSAGFGDSVVQYANSWVVVGAPQKITAANQTGGLYQCGYSTGACEPIGLQVPPEAVNMSLGLSLASTTSPSQLLACGPTVHHECGRNMYLTGLCFLLGPTQLTQRLPVSRQECPRQEQDIVFLIDGSGSISSRNFATMMNFVRAVISQFQRPSTQFSLMQFSNKFQTHFTFEEFRRSSNPLSLLASVHQLQGFTYTATAIQNVVHRLFHASYGARRDAAKILIVITDGKKEGDSLDYKDVIPMADAAGIIRYAIGVGLAFQNRNSWKELNDIASKPSQEHIFKVEDFDALKDIQNQLKEKIFAIEGTETTSSSSFELEMA

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 integrin, alpha X (complement component 3 receptor 4 subunit)
别名 2F1C10, CD11 antigen-like family member C, Integrin alpha X, Integrin alpha-X, ITGAX
计算分子量 1169 aa, 129 kDa
观测分子量 145 kDa
GenBank蛋白编号BC038237
基因名称 CD11c
Gene ID (NCBI) 3687
ENSEMBL Gene IDENSG00000140678
RRIDAB_2881379
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP20702
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis.

实验方案

Product Specific Protocols
IF protocol for CD11c antibody 60258-1-IgDownload protocol
IHC protocol for CD11c antibody 60258-1-IgDownload protocol
WB protocol for CD11c antibody 60258-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

Cell

Tissue-resident FOLR2+ macrophages associate with CD8+ T cell infiltration in human breast cancer.

Authors - Rodrigo Nalio Ramos
View Article
humanIF

Sci Immunol

Initiator cell death event induced by SARS-CoV-2 in the human airway epithelium

Authors - Kaixin Liang
View Article

MedComm (2020)

Plasmacytoid and CD141+ Myeloid Dendritic Cells Cooperation with CD8+ T Cells in Lymph Nodes is Associated with HIV Control

Authors - Joana Vitallé
View Article
humanIHC

Med

CCL19+ dendritic cells potentiate clinical benefit of anti-PD-(L)1 immunotherapy in triple-negative breast cancer

Authors - Song-Yang Wu
View Article
humanIF

J Exp Med

AIM2 regulates anti-tumor immunity and is a viable therapeutic target for melanoma.

Authors - Keitaro Fukuda
View Article
humanIF

J Exp Med

PMID: 32181805

Authors - Xiaoyan Zhan
View Article
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