• Featured Product
  • KD/KO验证

Integrin alpha-5/CD49e Polyclonal antibody

Integrin alpha-5/CD49e Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 27224-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA

Integrin Alpha-5, ITGA5, CD49 antigen-like family member E, CD49e, FNRA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inA549 cells, HAP1 cells, HeLa cells, human placenta tissue, U2OS cells
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ITGA5 Ready-To-Use IHC Kit. ITGA5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27224-1-AP targets Integrin alpha-5/CD49e in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原 Integrin alpha-5/CD49e fusion protein Ag26098 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
别名 Integrin Alpha-5, ITGA5, CD49 antigen-like family member E, CD49e, FNRA
计算分子量 115 kDa
观测分子量 135-150 kDa
GenBank蛋白编号NM_002205
基因名称 Integrin alpha 5
Gene ID (NCBI) 3678
RRIDAB_2880807
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Integrin alpha-5/CD49e antibody 27224-1-APDownload protocol
IHC protocol for Integrin alpha-5/CD49e antibody 27224-1-APDownload protocol
IP protocol for Integrin alpha-5/CD49e antibody 27224-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

J Clin Transl Hepatol

A Prognostic Nomogram for Hepatocellular Carcinoma Based on Wound Healing and Immune Checkpoint Genes

Authors - Beiyuan Hu
humanWB

Mol Cancer

ZNF460-mediated circRPPH1 promotes TNBC progression through ITGA5-induced FAK/PI3K/AKT activation in a ceRNA manner

Authors - Chuanpeng Zhang
humanWB

Biomater Res

Targeting Reprogrammed Cancer-Associated Fibroblasts with Engineered Mesenchymal Stem Cell Extracellular Vesicles for Pancreatic Cancer Treatment

Authors - Pengcheng Zhou
  • KD Validated
Loading...