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验证数据展示

  • WB analysis using 87417-1-RR (same clone as 87417-1-PBS)

    WB analysis using 87417-1-RR (same clone as 87417-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87417-1-RR (ITAE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87417-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 87417-1-RR (same clone as 87417-1-PBS)

    IHC staining of mouse spleen using 87417-1-RR (same clone as 87417-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 87417-1-RR (CD103 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87417-1-PBS in a different storage buffer formulation.

Mouse CD103 Recombinant monoclonal antibody, PBS Only

CD103 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 87417-1-PBS

产品说明书

CloneNo. 252752B6

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, Indirect ELISA

别名

Itgae, alpha E1, alpha M290, aM290, Integrin alpha E

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87417-1-PBS targets CD103 in WB, IHC, Indirect ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg10465

Product name: Recombinant mouse ITAE protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-Twin-Strep-Tag

Domain: 669-1114 aa of

Sequence: RPVVDLTVSMTFTPDALPMVFIGKMDVKLCFEVDSSGVASEPGLREMFLNFTVDVDVTKQRQRLQCEDSSGCQSCLRKWNGGSFLCEHFWLISTEELCEEDCFSNITIKVTYEFQTSGGRRDYPNPTLDHYKEPSAIFQLPYEKDCKNKVFCIAEIQLTTNISQQELVVGVTKEVTMNISLTNSGEDSYMTNMALNYPRNLQFKKIQKPVSPDVQCDDPKPVASVLVMNCKIGHPILKRSSVNVSVTWQLEESVFPNRTADITVTISNSNEKSLARETRSLQFRHAFIAVLSRPSVMYMNTSQSPSDHKEFFFNVHGENLFGAVFQLQICVPIKLQDFQIVRVKNLTKTQDHTECTQSQEPACGSDPVQHVKEWHSVVCAITSNKENVTVAAEISVGHTKQLLRDVSELPILGEISFNKSLYEGLNAENHRTKITVIFLKEEETRS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 integrin alpha E, epithelial-associated
别名 Itgae, alpha E1, alpha M290, aM290, Integrin alpha E
观测分子量160 kDa
基因名称 CD103
Gene ID (NCBI) 16407
RRIDAB_3745529
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD103, also known as integrin alpha-E (ITGAE) or integrin alpha-IEL, is a type I transmembrane integrin protein that binds integrin beta 7 to form Integrin alpha-E beta-7 which is a receptor for E-cadherin and mediates adhesion of T lymphocytes to epithelial cells (PMID: 7969453; 8468482). CD103 is expressed on intraepithelial lymphocyte (IEL) T cells (both alpha/beta T cells and gamma/delta T cells), some peripheral regulatory T cells (Tregs), lamina propria T cells, and a subset of dendritic cells in the gut mucosa and mesenteric lymph nodes (PMID: 12242333; 15608520; 16216890).

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