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ISOC1 Polyclonal antibody

ISOC1 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 25844-1-AP

Print datasheet

Synonyms

CGI 111, ISOC1

验证数据展示

  • WB analysis using 25844-1-AP

    WB analysis using 25844-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25844-1-AP (ISOC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 25844-1-AP

    IP experiment of mouse liver using 25844-1-AP

    IP result of anti-ISOC1 (IP:25844-1-AP, 4ug; Detection:25844-1-AP 1:300) with mouse liver tissue lysate 4000ug.

经过测试的应用

Positive WB detected inHT-29 cells, mouse liver tissue, HepG2 cells, SH-SY5Y cells, mouse embryo tissue
Positive IP detected inmouse liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25844-1-AP is ISOC1 Fusion Protein expressed in E. coli.

Tested Applications IP, WB,ELISA
Tested Reactivity human, mouse
Immunogen ISOC1 fusion protein Ag22611 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name isochorismatase domain containing 1
Synonyms CGI 111, ISOC1
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC014105
Gene Symbol ISOC1
Gene ID (NCBI) 51015
RRIDAB_2918095
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96CN7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ISOC1 antibody 25844-1-APDownload protocol
IP protocol for ISOC1 antibody 25844-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols