验证数据展示
经过测试的应用
| Positive WB detected in | HT-29 cells, TT cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30152-1-AP targets ISM1 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 | ISM1 fusion protein Ag32761 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | isthmin 1 homolog (zebrafish) |
| 别名 | bA149I18.1, C20orf82, dJ1077I2.1, ISM, ISM1, Isthmin, Isthmin 1, isthmin 1 homolog (zebrafish) |
| 计算分子量 | 52kd |
| 观测分子量 | 52 kDa, 70 kDa |
| GenBank蛋白编号 | NM_080826 |
| 基因名称 | ISM1 |
| Gene ID (NCBI) | 140862 |
| RRID | AB_3669703 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | B1AKI9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ISM1, also known as C20orf82. It is expected to be located in extracellular space, and the protein is enriched in the thyroid gland tissue. Acts as an angiogenesis inhibitor. The protein is predicted to be involved in negative regulation of angiogenesis. Since the molecular weight of the detected ISM1 was larger than expected (52 kD), ISM1 is likely subject to posttranslational modifications. Glycosylation is commonly found in many secreted proteins, and analysis of the ISM1 protein sequence predicted two putative N-glycosylation sites at positions N39 and N282. (PMID: 31171630)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ISM1 antibody 30152-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |