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Validation Data Gallery

  • ISG15-Trap magnetic agarose (MG-3001MA) was used to immunoprecipitate endogenous ISG15 and ISGylated proteins from untreated human HEPG2 cells (baseline) and interferon-β treated HepG2 cells (induced). Lysis was achieved with RIPA buffer. For each IP, samples of the input lysate (I), non-bound flow-through (FT), and bound (B) fractions were analyzed via Coomassie stained SDS-PAGE & Western blot. Anti-ISG15 polyclonal antibody (15981-1-AP) Multi-rAb® HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) were used in the Western blot analysis. The Trap shows efficient IP of endogenous ISG15 and ISGylated proteins with low background.

    ISG15-Trap magnetic agarose (MG-3001MA) was used to immunoprecipitate endogenous ISG15 and ISGylated proteins from untreated human HEPG2 cells (baseline) and interferon-β treated HepG2 cells (induced). Lysis was achieved with RIPA buffer. For each IP, samples of the input lysate (I), non-bound flow-through (FT), and bound (B) fractions were analyzed via Coomassie stained SDS-PAGE & Western blot. Anti-ISG15 polyclonal antibody (15981-1-AP) Multi-rAb® HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) were used in the Western blot analysis. The Trap shows efficient IP of endogenous ISG15 and ISGylated proteins with low background.

  • ISG15-Trap magnetic agarose (MG-3001MA) was used to immunoprecipitate endogenous ISG15 and ISGylated proteins from untreated human HEPG2 cells (baseline) and interferon-β treated HepG2 cells (induced). Lysis was achieved with standard Lysis buffer. For each IP, samples of the input lysate (I), non-bound flow-through (FT), and bound (B) fractions were analyzed via Coomassie stained SDS-PAGE & Western blot. Anti-ISG15 polyclonal antibody (15981-1-AP) and Multi-rAb® HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) were used in the Western blot analysis. The Trap shows efficient IP of endogenous ISG15 and ISGylated proteins with low background.

    ISG15-Trap magnetic agarose (MG-3001MA) was used to immunoprecipitate endogenous ISG15 and ISGylated proteins from untreated human HEPG2 cells (baseline) and interferon-β treated HepG2 cells (induced). Lysis was achieved with standard Lysis buffer. For each IP, samples of the input lysate (I), non-bound flow-through (FT), and bound (B) fractions were analyzed via Coomassie stained SDS-PAGE & Western blot. Anti-ISG15 polyclonal antibody (15981-1-AP) and Multi-rAb® HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) were used in the Western blot analysis. The Trap shows efficient IP of endogenous ISG15 and ISGylated proteins with low background.

ChromoTek ISG15-Trap Magnetic Agarose, Kit for Immunoprecipitation

The ChromoTek ISG15-Trap Magnetic Agarose, Kit for Immunoprecipitation consists of an anti-ISG15 VHH, which is coupled to magnetic agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of ISG15 tagged proteins from cell extracts of various organisms.
Cat No. MG-3001MAK

Reactivity

ISG15

Applications

IP, Co-IP

Host/Type

Alpaca, recombinant VHH

Conjugate

Magnetic Agarose

Synonyms

Interferon-induced 15 kDa protein, Interferon-induced 17 kDa protein (IP17), Ubiquitin cross-reactive protein (hUCRP), Ubiquitin-like protein ISG15

Formats:  Magnetic Agarose kit
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Product Information

The ChromoTek ISG15-Trap Magnetic Agarose, Kit for Immunoprecipitation consists of an anti-ISG15 VHH, which is coupled to magnetic agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of ISG15 tagged proteins from cell extracts of various organisms.

DescriptionThe ISG15-Trap Magnetic Agarose, Kit for Immunoprecipitation contains ISG15-Trap Magnetic Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of ISG15 proteins.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• Stable in harsh buffer conditions

• Suitable for downstream mass spec analysis

• High Specificity

• Low background

ApplicationsIP, Co-IP
Specificity/Target/
Binding Capacity5 μg of monovalent ISG15 (~17 kDa) per 25 μL bead slurry
Bead concentration6% Slurry
Resin TypeMagnetic Agarose beads; ~40 um (cross-linked 6% magnetic agarose beads)
Elution buffer2x SDS-sample buffer (Lämmli), 200 mM glycine pH 2.5
Wash Buffer Compatibility2M NaCl, 5 mM DTT, 5 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0.1% SDS, 1 M Urea
TypeVHH
ClassRecombinant - Animal free production
HostAlpaca
Affinity70 nM
Compatibility with mass spectrometryThe ISG15-Trap is optimized for on-bead digestion. For the application note, please click here:
On-bead digest protocol for mass spectrometry
RRIDAB_3740243
Storage Buffer20% Ethanol
Storage ConditionUpon arrival store at +4°C / do not freeze!
ShippingAmbient Temp

Kit components

Component Description
ISG15-Trap Magnetic Agarose20 reactions (500 µl)
Lysis bufferOptimized for cytoplasmic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash bufferRemoval of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution
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