验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, mouse cerebellum tissue, mouse heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20324-1-AP targets ISCA1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | ISCA1 fusion protein Ag14188 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | iron-sulfur cluster assembly 1 homolog (S. cerevisiae) |
| 别名 | RP11 507D14.2, ISA1, hIscA, HBLD2 |
| 计算分子量 | 129 aa, 14 kDa |
| 观测分子量 | 15 kDa |
| GenBank蛋白编号 | BC002675 |
| 基因名称 | ISCA1 |
| Gene ID (NCBI) | 81689 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BUE6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ISCA1, also named as HBLD2 and hIscA, belongs to the hesB/iscA family. It is involved in the assembly of mitochondrial iron-sulfur proteins. ISCA1 probably involved in the binding of an intermediate of Fe/S cluster assembly. ISCA1 plays an important role in both mitochondrial and cytosolic iron-sulfur cluster biogenesis, and a notable component of the latter is the interaction between ISCA1 and IOP1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ISCA1 antibody 20324-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |