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验证数据展示

  • WB analysis using 67142-1-Ig

    WB analysis using 67142-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67142-1-Ig (IRF8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 67142-1-Ig

    IF Staining of THP-1 using 67142-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using IRF8 antibody (67142-1-Ig, Clone: 3A10A4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

IRF8 Monoclonal antibody

IRF8 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67142-1-Ig

产品说明书

CloneNo. 3A10A4

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

3A10A4, H-ICSBP, ICSBP1, Interferon consensus sequence-binding protein, interferon regulatory factor 8

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 594
规格价格库存

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经过测试的应用

Positive WB detected inRamos cells, Raji cells, Daudi cells
Positive IF/ICC detected inTHP-1 cells
Planning an IF experiment? We recommend our CoraLite® Plus 594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67142-1-Ig targets IRF8 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19909

Product name: Recombinant human IRF8 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 121-426 aa of BC126247

Sequence: KLGVATAGCVNEVTEMECGRSEIDELIKEPSVDDYMGMIKRSPSPPEACRSQLLPDWWAQQPSTGVPLVTGYTTYDAHHSAFSQMVISFYYGGKLVGQATTTCPEGCRLSLSQPGLPGTKLYGPEGLELVRFPPADAIPSERQRQVTRKLFGHLERGVLLHSSRQGVFVKRLCQGRVFCSGNAVVCKGRPNKLERDEVVQVFDTSQFFRELQQFYNSQGRLPDGRVVLCFGEEFPDMAPLRSKLILVQIEQLYVRQLAEEAGKSCGAGSVMQAPEEPPPDQVFRMFPDICASHQRSFFRENQQITV

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 ICSBP1
别名 3A10A4, H-ICSBP, ICSBP1, Interferon consensus sequence-binding protein, interferon regulatory factor 8
计算分子量 426 aa, 48 kDa
观测分子量 48 kDa
GenBank蛋白编号BC126247
基因名称 IRF8
Gene ID (NCBI) 3394
RRIDAB_2882441
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ02556
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IRF8 also named as ICSBP1, is a transcription factor of the IFN regulatory factor (IRF) family. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection. IRF8 specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the IFN consensus sequence (ICS)). It plays a negative regulatory role in cells of the immune system. It is predominantly in lymphoid tissues.

实验方案

Product Specific Protocols
IF protocol for IRF8 antibody 67142-1-IgDownload protocol
WB protocol for IRF8 antibody 67142-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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