• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

IRF8 Monoclonal antibody

IRF8 Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A10A4

Cat No : 67142-1-Ig

Print datasheet

Synonyms

H ICSBP, ICSBP, ICSBP1, IRF 8, IRF8

验证数据展示

  • WB analysis using 67142-1-Ig

    WB analysis using 67142-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67142-1-Ig (IRF8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 67142-1-Ig

    IF Staining of THP-1 using 67142-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using IRF8 antibody (67142-1-Ig, Clone: 3A10A4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inRamos cells, Raji cells, Daudi cells
Positive IF detected inTHP-1 cells
Planning an IF experiment? We recommend our CoraLite® Plus 594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67142-1-Ig targets IRF8 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human
Immunogen IRF8 fusion protein Ag19909 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name ICSBP1
Synonyms H ICSBP, ICSBP, ICSBP1, IRF 8, IRF8
Calculated Molecular Weight 426 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC126247
Gene Symbol IRF8
Gene ID (NCBI) 3394
RRIDAB_2882441
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ02556
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IRF8 also named as ICSBP1, is a transcription factor of the IFN regulatory factor (IRF) family. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection. IRF8 specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the IFN consensus sequence (ICS)). It plays a negative regulatory role in cells of the immune system. It is predominantly in lymphoid tissues.

实验方案

Product Specific Protocols
WB protocol for IRF8 antibody 67142-1-IgDownload protocol
IF protocol for IRF8 antibody 67142-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols