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IRF3 Recombinant antibody

IRF3 Recombinant Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, Zebrafish

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2H6

Cat No : 80519-1-RR

Print datasheet

Synonyms

interferon regulatory factor 3, IRF 3, IRF3

验证数据展示

  • WB analysis using 80519-1-RR

    WB analysis using 80519-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80519-1-RR (IRF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of RAW 264.7 using 80519-1-RR

    WB analysis of RAW 264.7 using 80519-1-RR

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 80519-1-RR (IRF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 80519-1-RR

    IHC staining of human breast cancer using 80519-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80519-1-RR (IRF3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IRF3 antibody included
  • IF Staining of HeLa using 80519-1-RR

    IF Staining of HeLa using 80519-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using IRF3 antibody (80519-1-RR, Clone: 2H6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, THP-1 cells, HT-29 cells, RAW 264.7 cells, zebrafish tissue, HepG2 cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy IRF3 Ready-To-Use IHC Kit. IRF3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80519-1-RR targets IRF3 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, Zebrafish samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, mouse, Zebrafish
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interferon regulatory factor 3
Synonyms interferon regulatory factor 3, IRF 3, IRF3
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC009395
Gene Symbol IRF3
Gene ID (NCBI) 3661
RRIDAB_2918899
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14653
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes.

实验方案

Product Specific Protocols
WB protocol for IRF3 antibody 80519-1-RRDownload protocol
IHC protocol for IRF3 antibody 80519-1-RRDownload protocol
IF protocol for IRF3 antibody 80519-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols