验证数据展示
经过测试的应用
| Positive WB detected in | HT-29 cells, LoVo cells, A549 cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells |
| Positive IF/ICC detected in | U2OS cells, A549 cells |
Planning an IHC experiment? We recommend our IHCeasy IRF2BP2 Ready-To-Use IHC Kit. IRF2BP2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
61030-1-Ig targets IRF2BP2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | interferon regulatory factor 2 binding protein 2 |
| 别名 | Interferon regulatory factor 2-binding protein 2, IRF 2BP2, IRF2BP 2 |
| 计算分子量 | 61 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | NM_182972 |
| 基因名称 | IRF2BP2 |
| Gene ID (NCBI) | 359948 |
| RRID | AB_3743262 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q7Z5L9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IRF2BP2 belongs to the IRF2BP family. IRF2BP2 acts as a transcriptional repressor and as a transcriptional corepressor in a IRF2-dependent manner. This repression is not mediated at least in part by histone deacetylase activities.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for IRF2BP2 antibody 61030-1-Ig | Download protocol |
| WB protocol for IRF2BP2 antibody 61030-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
