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验证数据展示

  • WB analysis using 86048-1-RR

    WB analysis using 86048-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86048-1-RR (IRF2BP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86048-1-RR

    WB analysis using 86048-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86048-1-RR (IRF2BP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 86048-1-RR

    IF Staining of U2OS using 86048-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using IRF2BP1 antibody (86048-1-RR, Clone: 250630H5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

IRF2BP1 Recombinant antibody

IRF2BP1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86048-1-RR

产品说明书

CloneNo. 250630H5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

EC:2.3.2.27, Interferon regulatory factor 2-binding protein 1, IRF 2 binding protein 1, IRF 2BP1, IRF-2-binding protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, HEK-293 cells, Jurkar cells, rat brain tissue
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy IRF2BP1 Ready-To-Use IHC Kit. IRF2BP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86048-1-RR targets IRF2BP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 IRF2BP1 fusion protein Ag4596 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interferon regulatory factor 2 binding protein 1
别名 EC:2.3.2.27, Interferon regulatory factor 2-binding protein 1, IRF 2 binding protein 1, IRF 2BP1, IRF-2-binding protein 1
计算分子量 584 aa, 62 kDa
观测分子量65-70 kDa
GenBank蛋白编号BC038222
基因名称 IRF2BP1
Gene ID (NCBI) 26145
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8IU81
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IRF2BP1 inhibits IRF2-mediated transcriptional activation by interacting with IRF2. In epidermal growth factor receptor (EGFR) signaling, transient deSUMOylation of IRF2BP1 is essential for the proper expression of immediate early genes such as DUSP1 and ATF3. IRF2BP1 is also involved in the TGF-β signaling pathway by controlling the accumulation of cPML in the cytoplasm, which in turn affects the phosphorylation of Smad2 and the assembly of the Smad2-receptor complex.

实验方案

Product Specific Protocols
WB protocol for IRF2BP1 antibody 86048-1-RRDownload protocol
IF protocol for IRF2BP1 antibody 86048-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols