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IRF2BP1 Polyclonal antibody

IRF2BP1 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 13698-1-AP

Print datasheet

Synonyms

IRF 2 binding protein 1, IRF 2BP1, IRF2BP1

验证数据展示

  • IHC staining of human breast cancer using 13698-1-AP

    IHC staining of human breast cancer using 13698-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13698-1-AP (IRF2BP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IRF2BP1 antibody included
  • IHC staining of human breast cancer using 13698-1-AP

    IHC staining of human breast cancer using 13698-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13698-1-AP (IRF2BP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IRF2BP1 antibody included
  • WB analysis of HeLa using 13698-1-AP

    WB analysis of HeLa using 13698-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13698-1-AP (IRF2BP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 13698-1-AP

    IP experiment of HEK-293 using 13698-1-AP

    IP result of anti-IRF2BP1 (IP:13698-1-AP, 4ug; Detection:13698-1-AP 1:500) with HEK-293 cells lysate 2000ug.

  • IF Staining of HeLa using 13698-1-AP

    IF Staining of HeLa using 13698-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13698-1-AP (IRF2BP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, mouse brain tissue, rat lymph tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy IRF2BP1 Ready-To-Use IHC Kit. IRF2BP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13698-1-AP targets IRF2BP1 in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Cited ApplicationsWB, IF, ChIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen IRF2BP1 fusion protein Ag4596 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interferon regulatory factor 2 binding protein 1
Synonyms IRF 2 binding protein 1, IRF 2BP1, IRF2BP1
Calculated Molecular Weight 584 aa, 62 kDa
Observed Molecular Weight 62-72 kDa
GenBank Accession NumberBC038222
Gene Symbol IRF2BP1
Gene ID (NCBI) 26145
RRIDAB_2126853
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IU81
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IRF2BP1 antibody 13698-1-APDownload protocol
IHC protocol for IRF2BP1 antibody 13698-1-APDownload protocol
IF protocol for IRF2BP1 antibody 13698-1-APDownload protocol
IP protocol for IRF2BP1 antibody 13698-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF,ChIP

EMBO Rep

Transient deSUMOylation of IRF2BP proteins controls early transcription in EGFR signaling.

Authors - Sina V Barysch
View Article
humanWB

Cell Death Dis

The Sumo proteome of proliferating and neuronal-differentiating cells reveals Utf1 among key Sumo targets involved in neurogenesis.

Authors - Juan F Correa-Vázquez
View Article