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验证数据展示

  • IHC staining of human breast cancer using 13698-1-AP

    IHC staining of human breast cancer using 13698-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13698-1-AP (IRF2BP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IRF2BP1 antibody included
  • IHC staining of human breast cancer using 13698-1-AP

    IHC staining of human breast cancer using 13698-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13698-1-AP (IRF2BP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IRF2BP1 antibody included
  • WB analysis of HeLa using 13698-1-AP

    WB analysis of HeLa using 13698-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13698-1-AP (IRF2BP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 13698-1-AP

    IP experiment of HEK-293 using 13698-1-AP

    IP result of anti-IRF2BP1 (IP:13698-1-AP, 4ug; Detection:13698-1-AP 1:500) with HEK-293 cells lysate 2000ug.

  • IF Staining of HeLa using 13698-1-AP

    IF Staining of HeLa using 13698-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13698-1-AP (IRF2BP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IRF2BP1 Polyclonal antibody

IRF2BP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13698-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

别名

EC:2.3.2.27, Interferon regulatory factor 2-binding protein 1, IRF 2 binding protein 1, IRF 2BP1, IRF-2-binding protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, mouse brain tissue, rat lymph tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy IRF2BP1 Ready-To-Use IHC Kit. IRF2BP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13698-1-AP targets IRF2BP1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF, ChIP
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 IRF2BP1 fusion protein Ag4596 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interferon regulatory factor 2 binding protein 1
别名 EC:2.3.2.27, Interferon regulatory factor 2-binding protein 1, IRF 2 binding protein 1, IRF 2BP1, IRF-2-binding protein 1
计算分子量 584 aa, 62 kDa
观测分子量 62-72 kDa
GenBank蛋白编号BC038222
基因名称 IRF2BP1
Gene ID (NCBI) 26145
RRIDAB_2126853
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IU81
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IRF2BP1 inhibits IRF2-mediated transcriptional activation by interacting with IRF2. In epidermal growth factor receptor (EGFR) signaling, transient deSUMOylation of IRF2BP1 is essential for the proper expression of immediate early genes such as DUSP1 and ATF3. IRF2BP1 is also involved in the TGF-β signaling pathway by controlling the accumulation of cPML in the cytoplasm, which in turn affects the phosphorylation of Smad2 and the assembly of the Smad2-receptor complex.

实验方案

Product Specific Protocols
WB protocol for IRF2BP1 antibody 13698-1-APDownload protocol
IHC protocol for IRF2BP1 antibody 13698-1-APDownload protocol
IF protocol for IRF2BP1 antibody 13698-1-APDownload protocol
IP protocol for IRF2BP1 antibody 13698-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF,ChIP

EMBO Rep

Transient deSUMOylation of IRF2BP proteins controls early transcription in EGFR signaling.

Authors - Sina V Barysch
View Article
humanWB

Cell Death Dis

The Sumo proteome of proliferating and neuronal-differentiating cells reveals Utf1 among key Sumo targets involved in neurogenesis.

Authors - Juan F Correa-Vázquez
View Article