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验证数据展示

  • IHC staining of human breast cancer using 13698-1-AP

    IHC staining of human breast cancer using 13698-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13698-1-AP (IRF2BP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IRF2BP1 antibody included
  • IHC staining of human breast cancer using 13698-1-AP

    IHC staining of human breast cancer using 13698-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13698-1-AP (IRF2BP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IRF2BP1 antibody included
  • WB analysis of HeLa using 13698-1-AP

    WB analysis of HeLa using 13698-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13698-1-AP (IRF2BP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 13698-1-AP

    IP experiment of HEK-293 using 13698-1-AP

    IP result of anti-IRF2BP1 (IP:13698-1-AP, 4ug; Detection:13698-1-AP 1:500) with HEK-293 cells lysate 2000ug.

  • IF Staining of HeLa using 13698-1-AP

    IF Staining of HeLa using 13698-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13698-1-AP (IRF2BP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

IRF2BP1 Polyclonal antibody

IRF2BP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13698-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

别名

IRF-2BP1, IRF-2-binding protein 1, IRF 2BP1, IRF 2 binding protein 1, Interferon regulatory factor 2-binding protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, mouse brain tissue, rat lymph tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy IRF2BP1 Ready-To-Use IHC Kit. IRF2BP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13698-1-AP targets IRF2BP1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, ChIP, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 IRF2BP1 fusion protein Ag4596 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interferon regulatory factor 2 binding protein 1
别名 IRF-2BP1, IRF-2-binding protein 1, IRF 2BP1, IRF 2 binding protein 1, Interferon regulatory factor 2-binding protein 1
计算分子量 584 aa, 62 kDa
观测分子量 62-72 kDa
GenBank蛋白编号BC038222
基因名称 IRF2BP1
Gene ID (NCBI) 26145
RRIDAB_2126853
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IU81
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IRF2BP1 antibody 13698-1-APDownload protocol
IHC protocol for IRF2BP1 antibody 13698-1-APDownload protocol
IF protocol for IRF2BP1 antibody 13698-1-APDownload protocol
IP protocol for IRF2BP1 antibody 13698-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF,ChIP

EMBO Rep

Transient deSUMOylation of IRF2BP proteins controls early transcription in EGFR signaling.

Authors - Sina V Barysch
View Article
humanWB

Cell Death Dis

The Sumo proteome of proliferating and neuronal-differentiating cells reveals Utf1 among key Sumo targets involved in neurogenesis.

Authors - Juan F Correa-Vázquez
View Article