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验证数据展示

  • WB analysis using 67180-1-Ig

    WB analysis using 67180-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67180-1-Ig (IRAK4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse pancreas using 67180-1-Ig

    IHC staining of mouse pancreas using 67180-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67180-1-Ig (IRAK4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IRAK4 antibody included
  • IHC staining of human placenta using 67180-1-Ig

    IHC staining of human placenta using 67180-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67180-1-Ig (IRAK4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IRAK4 antibody included
  • IHC staining of human placenta using 67180-1-Ig

    IHC staining of human placenta using 67180-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67180-1-Ig (IRAK4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IRAK4 antibody included
  • IHC staining of mouse kidney using 67180-1-Ig

    IHC staining of mouse kidney using 67180-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67180-1-Ig (IRAK4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IRAK4 antibody included
  • IHC staining of rat pancreas using 67180-1-Ig

    IHC staining of rat pancreas using 67180-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 67180-1-Ig (IRAK4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IRAK4 antibody included
  • IHC staining of rat stomach using 67180-1-Ig

    IHC staining of rat stomach using 67180-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 67180-1-Ig (IRAK4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IRAK4 antibody included
  • IF Staining of HepG2 using 67180-1-Ig

    IF Staining of HepG2 using 67180-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IRAK4 antibody (67180-1-Ig, Clone: 1A4C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

IRAK4 Monoclonal antibody

IRAK4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67180-1-Ig

产品说明书

CloneNo. 1A4C4

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

1A4C4, EC:2.7.11.1, Interleukin-1 receptor-associated kinase 4, IPD1, IRAK 4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, THP-1 cells, K-562 cells
Positive IHC detected inhuman placenta tissue, mouse kidney tissue, mouse pancreas tissue, rat pancreas tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy® IRAK4 Ready-To-Use IHC Kit. IRAK4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67180-1-Ig targets IRAK4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag29174

Product name: Recombinant human IRAK4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-200 aa of BC013316

Sequence: MNKPITPSTYVRCLNVGLIRKLSDFIDPQEGWKKLAVAIKKPSGDDRYNQFHIRRFEALLQTGKSPTSELLFDWGTTNCTVGDLVDLLIQNEFFAPASLLLPDAVPKTANTLPSKEAITVQQKQMPFCDKDRTLMTPVQNLEQSYMPPDSSSPENKSLEVSDTRFHSFSFYELKNVTNNFDERPISVGGNKMGEGGFGVV

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin-1 receptor-associated kinase 4
别名 1A4C4, EC:2.7.11.1, Interleukin-1 receptor-associated kinase 4, IPD1, IRAK 4
计算分子量 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC013316
基因名称 IRAK4
Gene ID (NCBI) 51135
RRIDAB_2882476
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NWZ3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for IRAK4 antibody 67180-1-IgDownload protocol
IHC protocol for IRAK4 antibody 67180-1-IgDownload protocol
WB protocol for IRAK4 antibody 67180-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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