验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human lung cancer tissue |
Planning an IHC experiment? We recommend our IHCeasy® IRAK4 Ready-To-Use IHC Kit. IRAK4 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18221-1-AP targets IRAK4 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, sheep |
| 免疫原 |
CatNo: Ag12982 Product name: Recombinant human IRAK4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-200 aa of BC013316 Sequence: MNKPITPSTYVRCLNVGLIRKLSDFIDPQEGWKKLAVAIKKPSGDDRYNQFHIRRFEALLQTGKSPTSELLFDWGTTNCTVGDLVDLLIQNEFFAPASLLLPDAVPKTANTLPSKEAITVQQKQMPFCDKDRTLMTPVQNLEQSYMPPDSSSPENKSLEVSDTRFHSFSFYELKNVTNNFDERPISVGGNKMGEGGFGVV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | interleukin-1 receptor-associated kinase 4 |
| 别名 | EC:2.7.11.1, Interleukin-1 receptor-associated kinase 4, IPD1, IRAK 4, IRAK-4 |
| 计算分子量 | 52 kDa |
| 观测分子量 | 52 kDa |
| GenBank蛋白编号 | BC013316 |
| 基因名称 | IRAK4 |
| Gene ID (NCBI) | 51135 |
| RRID | AB_2878519 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NWZ3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for IRAK4 antibody 18221-1-AP | Download protocol |
| IHC protocol for IRAK4 antibody 18221-1-AP | Download protocol |
| IP protocol for IRAK4 antibody 18221-1-AP | Download protocol |
| WB protocol for IRAK4 antibody 18221-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep Med Integrated characterization of hepatobiliary tumor organoids provides a potential landscape of pharmacogenomic interactions | ||
Cancer Lett IL-1α facilitates GSH synthesis to counteract oxidative stress in oral squamous cell carcinoma under glucose-deprivation | ||
Int J Biol Sci BAP1 Represses Sequential Activation of IRAKs and NF-κB Signaling in Pancreatic Cancer | ||
Int J Biol Sci Targeting IL-1α Sensitizes HNSCC to PDT by Reversing Hypoxia and NF-κB-Driven Oxidative Stress Resistance. | ||
J Med Chem Design, Synthesis, and Evaluation of Nucleolin-Bridged, MDM2-Recruiting IRAK4 Degraders for the Treatment of Autoimmune Diseases. | ||
Int Immunopharmacol Herbal compound cepharanthine attenuates inflammatory arthritis by blocking macrophage M1 polarization |
