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验证数据展示

  • WB analysis of THP-1 using 83876-2-RR (same clone as 83876-2-PBS)

    WB analysis of THP-1 using 83876-2-RR (same clone as 83876-2-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83876-2-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 83876-2-RR (same clone as 83876-2-PBS)

    IF Staining of THP-1 using 83876-2-RR (same clone as 83876-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using IRAK3 antibody (83876-2-RR, Clone: 240975C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83876-2-PBS in a different storage buffer formulation.

  • WB analysis of mouse lung using 83876-2-RR (same clone as 83876-2-PBS)

    WB analysis of mouse lung using 83876-2-RR (same clone as 83876-2-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83876-2-PBS in a different storage buffer formulation.

  • WB analysis using 83876-2-RR (same clone as 83876-2-PBS)

    WB analysis using 83876-2-RR (same clone as 83876-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83876-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00849-2

    Cytometric bead array standard curve of MP00849-2

    Cytometric bead array standard curve of MP00849-2, IRAK3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83876-3-PBS. Detection antibody: 83876-2-PBS. Standard: Ag4959. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00849-3

    Cytometric bead array standard curve of MP00849-3

    Cytometric bead array standard curve of MP00849-3, IRAK3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83876-1-PBS. Detection antibody: 83876-2-PBS. Standard: Ag4959. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83876-2-RR

    Affinity and Kinetic Characterization of 83876-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83876-2-RR against Human IRAK3 were performed. The affinity constant is below 1 pM.

IRAK3 Recombinant antibody, PBS Only (Detector)

IRAK3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 83876-2-PBS

产品说明书

CloneNo. 240975C5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

IRAK 3, Interleukin-1 receptor-associated kinase 3, Inactive IL-1 receptor-associated kinase 3, IL-1 receptor-associated kinase M, ASRT5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy IRAK3 Ready-To-Use IHC Kit. IRAK3 primary antibody included.

产品信息

83876-2-PBS targets IRAK3 as part of a matched antibody pair:

MP00849-2: 83876-3-PBS capture and 83876-2-PBS detection (validated in Cytometric bead array)

MP00849-3: 83876-1-PBS capture and 83876-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 IRAK3 fusion protein Ag4959 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin-1 receptor-associated kinase 3
别名 IRAK 3, Interleukin-1 receptor-associated kinase 3, Inactive IL-1 receptor-associated kinase 3, IL-1 receptor-associated kinase M, ASRT5
计算分子量 68 kDa
观测分子量68 kDa
GenBank蛋白编号BC057800
基因名称 IRAK3
Gene ID (NCBI) 11213
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y616
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IRAK3 (Interleukin-1 receptor-associated kinase-3, also named IRAKM) as a kinase-deficient member of the TLR/IRAK family has been shown to negatively regulate Toll-like receptor (TLR)-mediated cell signaling (PMID: 33590831).