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IRAK3 Polyclonal antibody

IRAK3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 28766-1-AP

Print datasheet

Synonyms

ASRT5, FLJ13601, IRAK 3, IRAK M, IRAK3, IRAKM

验证数据展示

  • WB analysis using 28766-1-AP

    WB analysis using 28766-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28766-1-AP (IRAK3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 28766-1-AP

    IHC staining of mouse colon using 28766-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28766-1-AP (IRAK3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IRAK3 antibody included
  • IHC staining of rat small intestine using 28766-1-AP

    IHC staining of rat small intestine using 28766-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 28766-1-AP (IRAK3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IRAK3 antibody included

经过测试的应用

Positive WB detected inmouse spleen tissue, rat spleen tissue
Positive IHC detected inmouse colon tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IRAK3 Ready-To-Use IHC Kit. IRAK3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28766-1-AP targets IRAK3 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen IRAK3 fusion protein Ag30309 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interleukin-1 receptor-associated kinase 3
Synonyms ASRT5, FLJ13601, IRAK 3, IRAK M, IRAK3, IRAKM
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 60-65 kDa
GenBank Accession NumberBC057800
Gene Symbol IRAK3
Gene ID (NCBI) 11213
RRIDAB_2881211
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y616
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IRAK3 antibody 28766-1-APDownload protocol
IHC protocol for IRAK3 antibody 28766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols