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验证数据展示

  • IP experiment of mouse testis using 25605-1-AP (same clone as 25605-1-PBS)

    IP experiment of mouse testis using 25605-1-AP (same clone as 25605-1-PBS)

    IP result of anti-IQUB (IP:25605-1-AP, 4ug; Detection:25605-1-AP 1:500) with mouse testis tissue lysate 1120 ug. This data was developed using the same antibody clone with 25605-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse spleen using 25605-1-AP (same clone as 25605-1-PBS)

    IP experiment of mouse spleen using 25605-1-AP (same clone as 25605-1-PBS)

    IP result of anti-IQUB (IP:25605-1-AP, 4ug; Detection:25605-1-AP 1:500) with mouse spleen tissue lysate 1920 ug. This data was developed using the same antibody clone with 25605-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 25605-1-AP (same clone as 25605-1-PBS)

    IHC staining of mouse testis using 25605-1-AP (same clone as 25605-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25605-1-AP (IQUB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25605-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 25605-1-AP (same clone as 25605-1-PBS)

    IHC staining of mouse testis using 25605-1-AP (same clone as 25605-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25605-1-AP (IQUB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25605-1-PBS in a different storage buffer formulation.

IQUB Polyclonal antibody, PBS Only

IQUB Polyclonal Antibody for IHC, IP, Indirect ELISA
Cat No. 25605-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

25605-1-PBS targets IQUB in IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag19507

Product name: Recombinant human IQUB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC128181

Sequence: MSNQQEKYEAQNIVNSTEESDDAFDTVTIPVPSEEPQESDQTEEHESGIEQFSESHAIHVEEQSDQSFSSLEPDNEQLMEEVISPRQVSYTPQHHEKQYAMQRPNDDSLAFLDKIKSVKESLQESVEDSLATVKVVLIPVGQEIVIPFKVDTILKYLKDHFSHLLGIPHSVLQIRYSGKILKNNETLVQHGVKPQEIVQVEIFSTNPDLYPVRRIDGLTDVSQIITVTVQTGLDQYQQVPVEIVKSDFHKPFLGGFRHKVTGVEYHNAGTQTVPKRIPERLSIFCRDTQTVFQKKNLQQTTNTTSTQMTNIGVYVSNMTDKLVTPGKYFSAAEYHAQRLKAVIVIQTYYR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 IQ motif and ubiquitin domain containing
别名
计算分子量 791 aa, 93 kDa
观测分子量 70 kDa
GenBank蛋白编号BC128181
基因名称 IQUB
Gene ID (NCBI) 154865
RRIDAB_3085807
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NA54
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IQUB (IQ motif and ubiquitin domain containing) was discovered in 2002,4 which was located on chromosome 7q31.32 and its encoded protein mainly contained 2 domains, ubiquitin-like domain and IQ domain. IQUB had apparently higher expression in breast cancer than that in normal tissues, suggesting that it may act an important role in the occurrence and development of breast cancer (PMID: 29968965). IQUB is a critical RS1 adaptor in mouse sperm flagella, and is critical for sperm motility and fertilization (PMID: 36417862).

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