验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, MCF-7 cells, A549 cells, HEK-293 cells, HepG2 cells, Calu-1 cells |
| Positive IHC detected in | human placenta tissue, human stomach cancer tissue, rat small intestine tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy IQGAP3 Ready-To-Use IHC Kit. IQGAP3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25930-1-AP targets IQGAP3 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag23109 Product name: Recombinant human IQGAP3 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 530-880 aa of BC131536 Sequence: LINEALDKGSPEKTLSALLLPAAGLDDVSLPVAPRYHLLLVAAKRQKAQVTGDPGAVLWLEEIRQGVVRANQDTNTAQRMALGVAAINQAIKEGKAAQTERVLRNPAVALRGVVPDCANGYQRALESAMAKKQCPADTAFWVQHDMKDGTAYYFHLQTFQGIWEQPPGCPLNTSHLTREEIQSAVTKVTAAYDRQQLWKANVGFVIQLQARLRGFLVRQKFAEHSHFLRTWLPAVIKIQAHWRGYRQRKIYLEWLQYFKANLDAIIKIQAWARMWAARRQYLRRLHYFQKNVNSIVKIQAFFRARKAQDDYRILVHAPHPPLSVVRRFAHLLNQSQQDFLAEAELLKLQEE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | IQ motif containing GTPase activating protein 3 |
| 别名 | Ras GTPase-activating-like protein IQGAP3 |
| 计算分子量 | 1631 aa, 185 kDa |
| 观测分子量 | 185 kDa |
| GenBank蛋白编号 | BC131536 |
| 基因名称 | IQGAP3 |
| Gene ID (NCBI) | 128239 |
| RRID | AB_2880299 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86VI3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for IQGAP3 antibody 25930-1-AP | Download protocol |
| WB protocol for IQGAP3 antibody 25930-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis Intermediate filament IFFO1 negatively regulates the migration of lung cancer cells by inhibiting the IQGAP3-Cdc42 interaction | ||
Cancer Lett IQGAP3 interacts with Rad17 to recruit the Mre11-Rad50-Nbs1 complex and contributes to radioresistance in lung cancer. | ||
Clin Cancer Res Infiltrative vessel co-optive growth pattern induced by IQGAP3 overexpression promotes microvascular invasion in hepatocellular carcinoma | ||
Cell Mol Life Sci Targeting IQGAP3 skews macrophages polarization towards M1 phenotype and promotes antitumor immune response. | ||
Am J Pathol Focal Adhesion Kinase Activation Mediates IQ Motif-Containing GTPase-Activating Protein 3-Promoted Lung Metastasis in Osteosarcoma. | ||
Sci Rep IQGAP3 activates Hedgehog signaling to confer stemness and metastasis via up-regulating GLI1 in lung cancer
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