验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, A431 cells, MCF-7 cells, HEK-293 cells, HepG2 cells |
Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
25930-1-AP targets IQGAP3 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IF, IHC |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | IQGAP3 fusion protein Ag23109 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | IQ motif containing GTPase activating protein 3 |
别名 | IQGAP3 |
计算分子量 | 1631 aa, 185 kDa |
观测分子量 | 185 kDa |
GenBank蛋白编号 | BC131536 |
基因名称 | IQGAP3 |
Gene ID (NCBI) | 128239 |
RRID | AB_2880299 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q86VI3 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for IQGAP3 antibody 25930-1-AP | Download protocol |
IHC protocol for IQGAP3 antibody 25930-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Cancer Lett IQGAP3 interacts with Rad17 to recruit the Mre11-Rad50-Nbs1 complex and contributes to radioresistance in lung cancer. | ||
BMC Cancer Comprehensive analysis of the expression and prognosis for IQ motif-containing GTPase-activating proteins in hepatocellular carcinoma | ||
Biochim Biophys Acta Gen Subj Identification of the interactome of the DP1 receptor for Prostaglandin D2: Regulation of DP1 receptor signaling and trafficking by IQGAP1.
| ||
iScience Oncofetal protein IGF2BP1 regulates IQGAP3 expression to maintain stem cell potential in cancer | ||
PeerJ IQGAP3 in clear cell renal cell carcinoma contributes to drug resistance and genome stability
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Clin Cancer Res Infiltrative vessel co-optive growth pattern induced by IQGAP3 overexpression promotes microvascular invasion in hepatocellular carcinoma |