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验证数据展示

  • IHC staining of human colon cancer using 85651-1-RR

    IHC staining of human colon cancer using 85651-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85651-1-RR (IQGAP1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IQGAP1 antibody included
  • IF Staining of A431 using 85651-1-RR

    IF Staining of A431 using 85651-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using IQGAP1 antibody (85651-1-RR, Clone: 242206G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of MCF-7 using 85651-1-RR

    IF Staining of MCF-7 using 85651-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using IQGAP1 antibody (85651-1-RR, Clone: 242206G2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

IQGAP1 Recombinant monoclonal antibody

IQGAP1 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 85651-1-RR

产品说明书

CloneNo. 242206G2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

HUMORFA01, KIAA0051, p195, Ras GTPase-activating-like protein IQGAP1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, A431 cells
Planning an IHC experiment? We recommend our IHCeasy IQGAP1 Ready-To-Use IHC Kit. IQGAP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85651-1-RR targets IQGAP1 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19321

Product name: Recombinant human IQGAP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 478-827 aa of BC151834

Sequence: QLSSSVTGLTNIEEENCQRYLDELMKLKAQAHAENNEFITWNDIQACVDHVNLVVQEEHERILAIGLINEALDEGDAQKTLQALQIPAAKLEGVLAEVAQHYQDTLIRAKREKAQEIQDESAVLWLDEIQGGIWQSNKDTQEAQKFALGIFAINEAVESGDVGKTLSALRSPDVGLYGVIPECGETYHSDLAEAKKKKLAVGDNNSKWVKHWVKGGYYYYHNLETQEGGWDEPPNFVQNSMQLSREEIQSSISGVTAAYNREQLWLANEGLITRLQARCRGYLVRQEFRSRMNFLKKQIPAITCIQSQWRGYKQKKAYQDRLAYLRSHKDEVVKIQSLARMHQARKRYRD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 IQ motif containing GTPase activating protein 1
别名 HUMORFA01, KIAA0051, p195, Ras GTPase-activating-like protein IQGAP1
计算分子量 189 kDa
GenBank蛋白编号BC151834
基因名称 IQGAP1
Gene ID (NCBI) 8826
ENSEMBL Gene IDENSG00000140575
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP46940
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for IQGAP1 antibody 85651-1-RRDownload protocol
IHC protocol for IQGAP1 antibody 85651-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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