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验证数据展示

  • WB analysis using 22167-1-AP (same clone as 22167-1-PBS)

    WB analysis using 22167-1-AP (same clone as 22167-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 22167-1-AP (same clone as 22167-1-PBS)

    IHC staining of mouse kidney using 22167-1-AP (same clone as 22167-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IQGAP1 antibody included
  • IHC staining of mouse kidney using 22167-1-AP (same clone as 22167-1-PBS)

    IHC staining of mouse kidney using 22167-1-AP (same clone as 22167-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IQGAP1 antibody included
  • IP experiment of HeLa using 22167-1-AP (same clone as 22167-1-PBS)

    IP experiment of HeLa using 22167-1-AP (same clone as 22167-1-PBS)

    IP result of anti-IQGAP1 (IP:22167-1-AP, 4ug; Detection:22167-1-AP 1:8000) with HeLa cells lysate 1085 ug. This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 22167-1-PBS

    IF Staining of A549 using 22167-1-PBS

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using IQGAP1 antibody (22167-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of MCF-7 using 22167-1-AP (same clone as 22167-1-PBS)

    IF Staining of MCF-7 using 22167-1-AP (same clone as 22167-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using IQGAP1 antibody (22167-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 22167-1-AP (same clone as 22167-1-PBS)

    IF Staining of MCF-7 using 22167-1-AP (same clone as 22167-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using IQGAP1 antibody (22167-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 22167-1-AP (same clone as 22167-1-PBS)

    FC experiment of HeLa using 22167-1-AP (same clone as 22167-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug IQGAP1 Polyclonal antibody (22167-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 22167-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

IQGAP1 Polyclonal antibody, PBS Only

IQGAP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 22167-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

别名

HUMORFA01, KIAA0051, p195, Ras GTPase-activating-like protein IQGAP1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy IQGAP1 Ready-To-Use IHC Kit. IQGAP1 primary antibody included.

产品信息

22167-1-PBS targets IQGAP1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag17821

Product name: Recombinant human IQGAP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 478-827 aa of BC151834

Sequence: QLSSSVTGLTNIEEENCQRYLDELMKLKAQAHAENNEFITWNDIQACVDHVNLVVQEEHERILAIGLINEALDEGDAQKTLQALQIPAAKLEGVLAEVAQHYQDTLIRAKREKAQEIQDESAVLWLDEIQGGIWQSNKDTQEAQKFALGIFAINEAVESGDVGKTLSALRSPDVGLYGVIPECGETYHSDLAEAKKKKLAVGDNNSKWVKHWVKGGYYYYHNLETQEGGWDEPPNFVQNSMQLSREEIQSSISGVTAAYNREQLWLANEGLITRLQARCRGYLVRQEFRSRMNFLKKQIPAITCIQSQWRGYKQKKAYQDRLAYLRSHKDEVVKIQSLARMHQARKRYRD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 IQ motif containing GTPase activating protein 1
别名 HUMORFA01, KIAA0051, p195, Ras GTPase-activating-like protein IQGAP1
计算分子量 189 kDa
观测分子量 190-195 kDa
GenBank蛋白编号BC151834
基因名称 IQGAP1
Gene ID (NCBI) 8826
ENSEMBL Gene IDENSG00000140575
RRIDAB_10949193
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP46940
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IQGAP1 is a member of a family of scaffolding proteins that interact with signaling and structural molecules. It localizes to sites of cell-cell contact in epithelial cells and regulates distinct cellular processes including cell adhesion, cell migration, extracellular signals through interacting with numerous protein. Multiple studies have shown that IQGAP1 is up-regulated in many human malignancies, such as lung cancer, ovarian cancer, colon cancer, breast cancer, melanoma and HCC. And IQGAP1 plays a critical role in cancer cell invasion.

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