• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 32476-1-AP

    WB analysis using 32476-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32476-1-AP (IPO8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IPO8 Polyclonal antibody

IPO8 Polyclonal Antibody for WB, ELISA
Cat No. 32476-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

Imp8, Importin-8, Ran-binding protein 8, RanBP8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, MDA-MB-231 cells, NIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32476-1-AP targets IPO8 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 IPO8 fusion protein Ag35395 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 importin 8
别名 Imp8, Importin-8, Ran-binding protein 8, RanBP8
观测分子量120-125 kDa
GenBank蛋白编号BC167853
基因名称 IPO8
Gene ID (NCBI) 10526
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO15397
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IPO8(Importin 8) is a gene encoding protein, which is located on human chromosome 12. It belongs to the nuclear protein transport family, and is mainly involved in the process of nuclear protein introduction. IPO8 regulates the transport function of nuclear pore complex by interacting with Ran GTPase and other proteins (PMID: 11682607). It is expected to be located in the cytoplasm and nucleus. IPO8 is associated with many diseases, including VISS syndrome and Loeys-Dietz syndrome. These diseases usually involve cardiovascular defects, skeletal abnormalities and immune loss. The molecular weight of PO8 protein is about 125kDa, and its structure includes helix and β-sheet. It has a specific three-dimensional structure and can form complexes with other protein.

实验方案

Product Specific Protocols
WB protocol for IPO8 antibody 32476-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols