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IPO7 Monoclonal antibody

IPO7 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E7C12

Cat No : 67525-1-Ig

Print datasheet

Synonyms

FLJ14581, Imp7, importin 7, IPO7, Ran binding protein 7, RANBP7

验证数据展示

  • WB analysis using 67525-1-Ig

    WB analysis using 67525-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67525-1-Ig (IPO7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IHC staining of human breast cancer using 67525-1-Ig

    IHC staining of human breast cancer using 67525-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67525-1-Ig (IPO7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IPO7 antibody included
  • WB analysis using 67525-1-Ig

    WB analysis using 67525-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67525-1-Ig (IPO7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, U2OS cells, LNCaP cells, HeLa cells, HepG2 cells, K-562 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IPO7 Ready-To-Use IHC Kit. IPO7 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67525-1-Ig targets IPO7 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen IPO7 fusion protein Ag27257 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name importin 7
Synonyms FLJ14581, Imp7, importin 7, IPO7, Ran binding protein 7, RANBP7
Calculated Molecular Weight 120 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberNM_006391.2
Gene Symbol IPO7
Gene ID (NCBI) 10527
RRIDAB_2882745
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95373
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IPO7 antibody 67525-1-IgDownload protocol
IHC protocol for IPO7 antibody 67525-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols