• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67525-1-Ig

    WB analysis using 67525-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67525-1-Ig (IPO7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IHC staining of human breast cancer using 67525-1-Ig

    IHC staining of human breast cancer using 67525-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67525-1-Ig (IPO7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IPO7 antibody included
  • WB analysis using 67525-1-Ig

    WB analysis using 67525-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67525-1-Ig (IPO7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

IPO7 Monoclonal antibody

IPO7 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67525-1-Ig

产品说明书

CloneNo. 1E7C12
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

1E7C12, Imp7, importin 7, Ran binding protein 7, RANBP7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, U2OS cells, LNCaP cells, HeLa cells, HepG2 cells, K-562 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IPO7 Ready-To-Use IHC Kit. IPO7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67525-1-Ig targets IPO7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性rat
免疫原

CatNo: Ag27257

Product name: Recombinant human IPO7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 879-1016 aa of NM_006391.2

Sequence: MHAEHENDSDDDDEAEDDDETEELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQTIQNRNPVWYQALTHGLNEEQRKQLQDIATLADQRRAAHESKMIEKHGG

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 importin 7
别名 1E7C12, Imp7, importin 7, Ran binding protein 7, RANBP7
计算分子量 120 kDa
观测分子量 120 kDa
GenBank蛋白编号NM_006391.2
基因名称 IPO7
Gene ID (NCBI) 10527
RRIDAB_2882745
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO95373
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IPO7 antibody 67525-1-IgDownload protocol
IHC protocol for IPO7 antibody 67525-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Exp Neurol

SDF2L1 downregulation mediates high glucose-caused Schwann cell dysfunction by inhibiting nuclear import of TFEB and CREB via KPNA3

Authors - Tingting Jin
View Article