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验证数据展示

  • WB analysis of human adrenal gland using 13155-1-AP

    WB analysis of human adrenal gland using 13155-1-AP

    human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 13155-1-AP (IP6K2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 13155-1-AP

    IHC staining of human liver cancer using 13155-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13155-1-AP (IP6K2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP6K2 Polyclonal antibody

IP6K2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13155-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

IHPK2, InsP6 kinase 2, IP6K2, P(i) uptake stimulator, PiUS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman adrenal gland tissue, human heart tissue, K-562 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3600
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13155-1-AP targets IP6K2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 IP6K2 fusion protein Ag3786 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 inositol hexakisphosphate kinase 2
别名 IHPK2, InsP6 kinase 2, IP6K2, P(i) uptake stimulator, PiUS
计算分子量 426 aa, 49 kDa
观测分子量 49 kDa
GenBank蛋白编号BC019694
基因名称 IP6K2
Gene ID (NCBI) 51447
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHH9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IP6K2(Inositol hexakisphosphate kinase 2) is also named as IHPK2, PiUS and belongs to the inositol phosphokinase (IPK) family. It converts inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5) and and mediates apoptotic cell death via its translocation from the nucleus to the cytoplasm(PMID:21652713). IP6K2 regulates TP53 and protein kinase AKT signaling, and its aberrant increase in cells has been implicated in apoptosis and INS resistance(PMID:21262846). It has 6 isoforms produced by alternative splicing. This antibody recognizes the others except the 8 kDa isoform of IP6K2.

实验方案

Product Specific Protocols
WB protocol for IP6K2 antibody 13155-1-APDownload protocol
IHC protocol for IP6K2 antibody 13155-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols