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IP6K1 Polyclonal antibody

IP6K1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 12057-2-AP

Print datasheet

Synonyms

IHPK1, InsP6 kinase 1, IP6K1, KIAA0263, PiUS

验证数据展示

  • WB analysis using 12057-2-AP

    WB analysis using 12057-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12057-2-AP (IP6K1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 12057-2-AP

    IHC staining of human lung cancer using 12057-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12057-2-AP (IP6K1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 12057-2-AP

    IHC staining of human colon cancer using 12057-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12057-2-AP (IP6K1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 12057-2-AP

    IHC staining of human colon cancer using 12057-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12057-2-AP (IP6K1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse kidney using 12057-2-AP

    IP experiment of mouse kidney using 12057-2-AP

    IP result of anti-IP6K1 (IP:12057-2-AP, 4ug; Detection:12057-2-AP 1:300) with mouse kidney tissue lysate 3000 ug.

经过测试的应用

Positive WB detected inA375 cells, human liver tissue, human kidney tissue, Hela cells, HepG2 cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12057-2-AP targets IP6K1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitypig
Immunogen IP6K1 fusion protein Ag2687 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name inositol hexakisphosphate kinase 1
Synonyms IHPK1, InsP6 kinase 1, IP6K1, KIAA0263, PiUS
Calculated Molecular Weight 441 aa, 50 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC012944
Gene Symbol IP6K1
Gene ID (NCBI) 9807
RRIDAB_2127529
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92551
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IP6K1 antibody 12057-2-APDownload protocol
IHC protocol for IP6K1 antibody 12057-2-APDownload protocol
IP protocol for IP6K1 antibody 12057-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

Pediatr Res

Preterm birth alters the feeding-induced activation of Akt signaling in the muscle of neonatal piglets

Authors - Agus Suryawan
View Article