INTS9 Polyclonal antibody

INTS9 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 11657-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA

RC 74, INTS 9, Int9, CPSF2L, CPSF2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11657-1-AP targets INTS9 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IP
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag2226

Product name: Recombinant human INTS9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 309-658 aa of BC025267

Sequence: LECLYQYIDSAGLSSVPLYFISPVANSSLEFSQIFAEWLCHNKQSKVYLPEPPFPHAELIQTNKLKHYPSIHGDFSNDFRQPCVVFTGHPSLRFGDVVHFMELWGKSSLNTVIFTEPDFSYLEALAPYQPLAMKCIYCPIDTRLNFIQVSKLLKEVQPLHVVCPEQYTQPPPAQSHRMDLMIDCQPPAMSYRRAEVLALPFKRRYEKIEIMPELADSLVPMEIKPGISLATVSAVLHTKDNKHLLQPPPRPAQPTSGKKRKRVSDDVPDCKVLKPLLSGSIPVEQFVQTLEKHGFSDIKVEDTAKGHIVLLQEAETLIQIEEDSTHIICDNDEMLRVRLRDLVLKFLQKF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 integrator complex subunit 9
别名 RC 74, INTS 9, Int9, CPSF2L, CPSF2
计算分子量 658 aa, 74 kDa
观测分子量 74 kDa
GenBank蛋白编号BC025267
基因名称 INTS9
Gene ID (NCBI) 55756
RRIDAB_2127514
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NV88
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INTS9, also known as Integrator Complex Subunit 9, is part of the core cleavage module consisting of INTS4/INTS9/INTS11 involved in processing small nuclear RNA (snRNA) molecules. It is a subunit of the Integrator complex, which comprises 15 subunits (including INTS6L), and plays a crucial role in the 3' end processing of snRNAs. By participating in the biogenesis of snRNAs, INTS9 contributes to the proper functioning of the spliceosome, a cellular machinery responsible for the accurate splicing of precursor messenger RNA (pre-mRNA) molecules. This process is essential for generating mature messenger RNA (mRNA), which is subsequently translated into functional proteins.

实验方案

Product Specific Protocols
WB protocol for INTS9 antibody 11657-1-APDownload protocol
IHC protocol for INTS9 antibody 11657-1-APDownload protocol
IP protocol for INTS9 antibody 11657-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Assembly mechanism of Integrator's RNA cleavage module

Authors - Kevin Sabath
humanWB

Nat Commun

INTS10-INTS13-INTS14 form a functional module of Integrator that binds nucleic acids and the cleavage module.

Authors - Kevin Sabath
humanWB

Protein Expr Purif

CRISPR-Cas9 mediated genetic engineering for the purification of the endogenous integrator complex from mammalian cells.

Authors - David Baillat
humanWB,IP

Mol Cell

Integrator facilitates RNAPII removal to prevent transcription-replication collisions and genome instability

Authors - Rahul Bhowmick
humanWB,IP

Mol Cell

Cytoplasmic binding partners of the Integrator endonuclease INTS11 and its paralog CPSF73 are required for their nuclear function

Authors - Min-Han Lin
humanWB

Elife

Human promoter directionality is determined by transcriptional initiation and the opposing activities of INTS11 and CDK9

Authors - Joshua D Eaton
Loading...