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验证数据展示

  • WB analysis of HepG2 using 83590-6-RR (same clone as 83590-6-PBS)

    WB analysis of HepG2 using 83590-6-RR (same clone as 83590-6-PBS)

    WB result of INTS4 antibody (83590-6-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-INTS4 transfected HepG2 cells. This data was developed using the same antibody clone with 83590-6-PBS in a different storage buffer formulation.

  • WB analysis using 83590-6-RR (same clone as 83590-6-PBS)

    WB analysis using 83590-6-RR (same clone as 83590-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83590-6-RR (INTS4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83590-6-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 83590-6-RR (same clone as 83590-6-PBS)

    IHC staining of human ovary cancer using 83590-6-RR (same clone as 83590-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83590-6-RR (INTS4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83590-6-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 83590-6-RR (same clone as 83590-6-PBS)

    IF Staining of U2OS using 83590-6-RR (same clone as 83590-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using INTS4 antibody (83590-6-RR, Clone: 240579F7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83590-6-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83590-6-RR (same clone as 83590-6-PBS)

    IF Staining of HeLa using 83590-6-RR (same clone as 83590-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using INTS4 antibody (83590-6-RR, Clone: 240579F7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83590-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83590-6-RR

    Affinity and Kinetic Characterization of 83590-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83590-6-RR against Human INTS4 were performed. The affinity constant is 0.149 nM.

  • Featured Product
  • KD/KO验证

INTS4 Recombinant monoclonal antibody, PBS Only

INTS4 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83590-6-PBS

产品说明书

CloneNo. 240579F7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

240579F7, Int4, MST093, MSTP093

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83590-6-PBS targets INTS4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag9116

Product name: Recombinant human INTS4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-331 aa of BC009995

Sequence: MAAHLKKRVYEEFTKVVQPQEEIATKKLRLTKPSKSAALHIDLCKATSPADALQYLLQFARKPVEAESVEGVVRILLEHYYKENDPSVRLKIASLLGLLSKTAGFSPDCIMDDAINILQNEKSHQVLAQLLDTLLAIGTKLPENQAIQMRLVDVACKHLTDTSHGVRNKCLQLLGNLGSLEKSVTKDAEGLAARDVQKIIGDYFSDQDPRVRTAAIKAMLQLHERGLKLHQTIYNQACKLLSDDYEQVRSAAVQLIWVVSQLYPESIVPIPSSNEEIRLVDDAFGKICHMVSDGSWVVRVQAAKLLGSMEQVSSHFLEQTLDKKLMSDLRR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 integrator complex subunit 4
别名 240579F7, Int4, MST093, MSTP093
计算分子量963aa,108 kDa; 505aa,57 kDa
观测分子量108 kDa
GenBank蛋白编号BC009995
基因名称 INTS4
Gene ID (NCBI) 92105
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ96HW7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Integrator complex subunit 4 (INTS4), a helical repeat protein, plays a key role in stabilizing nuclease domains and other components. INTS4 is a specific and conserved interaction partner of INTS9/11 heterodimer that does not interact with either subunit individually (PMID: 29471365). INTS4 plays a role in achieving a compact nuclease/pseudonuclease configuration resembling that observed for CPSF73/100 in the histone-processing machinery (PMID: 33548203).

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