验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
16455-1-AP targets INTS12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag9403 Product name: Recombinant human INTS12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 106-462 aa of BC014442 Sequence: SDITEGVDIPKKPRLEKPETQSSPITVQSSKDLPMADLSSFEETSADDFAMEMGLACVVCRQMMVASGNQLVECQECHNLYHRDCHKPQVTDKEANDPRLVWYCARCTRQMKRMAQKTQKPPQKPAPAVVSVTPAVKDPLVKKPETKLKQETTFLAFKRTEVKTSTVISGNSSSASVSSSVTSGLTGWAAFAAKTSSAGPSTAKLSSTTQNNTGKPATSSANQKPVGLTGLATSSKGGIGSKIGSNNSTTPTVPLKPPPPLTLGKTGLSRSVSCDNVSKVGLPSPSSLVPGSSSQLSGNGNSGTSGPSGSTTSKTTSESSSSPSASLKGPTSQESQLNAMKRLQMVKKKAAQKKLKK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | integrator complex subunit 12 |
| 别名 | Int12, PHF22, SBBI22 |
| 计算分子量 | 462 aa, 49 kDa |
| 观测分子量 | 49 kDa |
| GenBank蛋白编号 | BC014442 |
| 基因名称 | INTS12 |
| Gene ID (NCBI) | 57117 |
| RRID | AB_2127263 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96CB8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
INTS12 is a subunit of the Integrator complex that is thought to act as a reader to link the complex to chromatin.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for INTS12 antibody 16455-1-AP | Download protocol |
| IHC protocol for INTS12 antibody 16455-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Redundant pathways for removal of defective RNA polymerase II complexes at a promoter-proximal pause checkpoint | ||
Nat Commun INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex | ||
PLoS Genet Human mutations in integrator complex subunits link transcriptome integrity to brain development. | ||
Cell Rep A homozygous variant in INTS11 links mitosis and neurogenesis defects to a severe neurodevelopmental disorder |
