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INPP5K Polyclonal antibody

INPP5K Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 15098-1-AP

Print datasheet

Synonyms

INPP5K, PPS, SKIP

验证数据展示

  • WB analysis of HEK-293 using 15098-1-AP

    WB analysis of HEK-293 using 15098-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15098-1-AP (INPP5K antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 15098-1-AP

    IHC staining of human kidney using 15098-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15098-1-AP (INPP5K antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with INPP5K antibody included
  • IHC staining of human kidney using 15098-1-AP

    IHC staining of human kidney using 15098-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15098-1-AP (INPP5K antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with INPP5K antibody included
  • IF Staining of HEK-293 using 15098-1-AP

    IF Staining of HEK-293 using 15098-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 15098-1-AP (INPP5K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse heart tissue
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy INPP5K Ready-To-Use IHC Kit. INPP5K primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15098-1-AP targets INPP5K in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen INPP5K fusion protein Ag7192 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name inositol polyphosphate-5-phosphatase K
Synonyms INPP5K, PPS, SKIP
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 45-51 kDa
GenBank Accession NumberBC004362
Gene Symbol INPP5K
Gene ID (NCBI) 51763
RRIDAB_2126365
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BT40
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for INPP5K antibody 15098-1-APDownload protocol
IHC protocol for INPP5K antibody 15098-1-APDownload protocol
IF protocol for INPP5K antibody 15098-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Chem Biol

Triplexed Affinity Reagents to Sample the Mammalian Inositol Pyrophosphate Interactome.

Authors - David Furkert
View Article