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验证数据展示

  • WB analysis using 29949-1-AP

    WB analysis using 29949-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29949-1-AP (INPP5J antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 29949-1-AP

    IF Staining of HeLa using 29949-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using INPP5J antibody (29949-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

INPP5J Polyclonal antibody

INPP5J Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29949-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

EC:3.1.3.36, EC:3.1.3.56, Inositol polyphosphate 5-phosphatase J, INPP5, Phosphatidylinositol 1,3,4,5-tetrakisphosphate 5-phosphatase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells, mouse heart tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29949-1-AP targets INPP5J in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag29242

Product name: Recombinant human INPP5J protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-150 aa of BC109288

Sequence: MALPRLGTQSTGPGRCLSPNLQAQEAPAPVTTSSSTSTLSSSPWSAQPTWKSDPGFRITVVTWNVGTAMPPDDVTSLLHLGGGDDSDGADMIAIGLQEVNSMLNKRLKDALFTDQWSELFMDALGPFNFVLVSSVRMQGVILLLFAKYYH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 inositol polyphosphate-5-phosphatase J
别名 EC:3.1.3.36, EC:3.1.3.56, Inositol polyphosphate 5-phosphatase J, INPP5, Phosphatidylinositol 1,3,4,5-tetrakisphosphate 5-phosphatase
计算分子量 1006 aa, 107 kDa
观测分子量70 kDa
GenBank蛋白编号BC109288
基因名称 INPP5J
Gene ID (NCBI) 27124
RRIDAB_2918353
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15735
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INPP5J(Phosphatidylinositol 4,5-bisphosphate 5-phosphatase A) is also named as PIB5PA, PIPP and belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type II family. INPP5J is a key signalling molecule that regulates dendritic outgrowth through activation of small GTPase signalling via interaction between FRMPD4 and ARHGEF7. It can control the resting membrane potential through a specific ion-channel-receptor-ligand interaction that brings about a large conformational change, analogous to neurotransmitter activation of ion channels at synapses. (PMID:22595022). This protein has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for INPP5J antibody 29949-1-APDownload protocol
WB protocol for INPP5J antibody 29949-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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