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验证数据展示

  • IF Staining of hTERT-RPE1 using 82689-3-RR (same clone as 82689-3-PBS)

    IF Staining of hTERT-RPE1 using 82689-3-RR (same clone as 82689-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using INPP5E antibody (82689-3-RR, Clone: 1E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 82689-3-PBS in a different storage buffer formulation.

  • IF Staining of hTERT-RPE1 using 82689-3-RR (same clone as 82689-3-PBS)

    IF Staining of hTERT-RPE1 using 82689-3-RR (same clone as 82689-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using INPP5E antibody (82689-3-RR, Clone: 1E7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 82689-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82689-3-RR

    Affinity and Kinetic Characterization of 82689-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82689-3-RR against Human INPP5E were performed. The affinity constant is 0.166 nM.

INPP5E Recombinant monoclonal antibody, PBS Only

INPP5E Uni-rAb® Recombinant Antibody for IF/ICC, Indirect ELISA
Cat No. 82689-3-PBS

产品说明书

CloneNo. 1E7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, Indirect ELISA

别名

1E7, 72 kDa inositol polyphosphate 5-phosphatase, EC:3.1.3.36, EC:3.1.3.86, Inositol polyphosphate-5-phosphatase E

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82689-3-PBS targets INPP5E in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30305

Product name: Recombinant human INPP5E protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 184-350 aa of BC028032

Sequence: RLPSLLPPRPPPALSLDIASDSLRTANKVDSDLADYKLRAQPLLVRAHSSLGPGRPRSPLACDDCSLRSAKSSFSLLAPIRSKDVRSRSYLEGSLLASGALLGADELARYFPDRNVALFVATWNMQGQKELPPSLDEFLLPAEADYAQDLYVIGVQEGCSDRREWET

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 inositol polyphosphate-5-phosphatase, 72 kDa
别名 1E7, 72 kDa inositol polyphosphate 5-phosphatase, EC:3.1.3.36, EC:3.1.3.86, Inositol polyphosphate-5-phosphatase E
计算分子量 644 aa, 70 kDa
观测分子量 64-66 kDa
GenBank蛋白编号BC028032
基因名称 INPP5E
Gene ID (NCBI) 56623
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NRR6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

INPP5E(72 kDa inositol polyphosphate 5-phosphatase) converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns-P2.In mouse, highest protein expression was in brain, heart, and testis, with lower expression in thymus and lung, and very little expression in kidney, spleen, and liver(PMID:10764818). Defects in INPP5E are the cause of Joubert syndrome type 1 (JBTS1) and mental retardation-truncal obesity-retinal dystrophy-micropenis (MORMS)(PMID:19668215). It has 2 isoforms with the molecular mass of 66 kDa and 70 kDa.