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验证数据展示

  • WB analysis of SH-SY5Y using 17797-1-AP

    WB analysis of SH-SY5Y using 17797-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17797-1-AP (INPP5E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 17797-1-AP

    IHC staining of human testis using 17797-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17797-1-AP (INPP5E antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 17797-1-AP

    IHC staining of human testis using 17797-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17797-1-AP (INPP5E antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of hTERT-RPE1 cells using 17797-1-AP

    IF Staining of hTERT-RPE1 cells using 17797-1-AP

    IF result of ciliary INPP5E in human hTERT-RPE1 cell: Upper is from control siRNA transfected cells and bottom is from INPP5E siRNA transfected cells. 17797-1-AP specifically recognizes the ciliary INPP5E (From Dr.Seongjin Seo).

  • IP experiment of HEK-293 using 17797-1-AP

    IP experiment of HEK-293 using 17797-1-AP

    IP result of anti-INPP5E (IP:17797-1-AP, 3ug; Detection:17797-1-AP 1:1000) with HEK-293 cells lysate 1000ug.

  • IF Staining of hTERT-RPE1 using 17797-1-AP

    IF Staining of hTERT-RPE1 using 17797-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using INPP5E antibody (17797-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of hTERT-RPE1 using 17797-1-AP

    IF Staining of hTERT-RPE1 using 17797-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using INPP5E antibody (17797-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • Featured Product
  • KD/KO Validated

INPP5E Polyclonal antibody

INPP5E Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 17797-1-AP

产品说明书

引用文献(62)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

别名

72 kDa inositol polyphosphate 5-phosphatase, EC:3.1.3.36, EC:3.1.3.86, Inositol polyphosphate-5-phosphatase E, Phosphatidylinositol 4,5-bisphosphate 5-phosphatase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inSH-SY5Y cells, HEK-293 cells, mouse brain tissue, mouse testis tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman testis tissue, human kidney tissue, human placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17797-1-AP targets INPP5E in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, zebrafish
免疫原

CatNo: Ag11959

Product name: Recombinant human INPP5E protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC028032

Sequence: MPSKAENLRPSEPAPQPPEGRTLQGQLPGAPPAQRAGSPPDAPGSESPALACSTPATPSGEDPPARAAPIAPRPPARPRLERALSLDDKGWRRRRFRGSQEDLEARNGTSPSRGSVQSEGPGAPAHSCSPPCLSTSLQEIPKSRGVLSSERGSPSSGGNPLSGVASSSPNLPHRDAAVAGSSPRLPSLLPPRPPPALSLDIASDSLRTANKVDSDLADYKLRAQPLLVRAHSSLGPGRPRSPLACDDCSLRSAKSSFSLLAPIRSKDVRSRSYLEGSLLASGALLGADELARYFPDRNVALFVATWNMQGQKELPPSLDEFLLPAEADYAQDLYVIGVQEGCSDRREWET

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 inositol polyphosphate-5-phosphatase, 72 kDa
别名 72 kDa inositol polyphosphate 5-phosphatase, EC:3.1.3.36, EC:3.1.3.86, Inositol polyphosphate-5-phosphatase E, Phosphatidylinositol 4,5-bisphosphate 5-phosphatase
计算分子量 644 aa, 70 kDa
观测分子量 64-66 kDa
GenBank蛋白编号BC028032
基因名称 INPP5E
Gene ID (NCBI) 56623
RRIDAB_2167120
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRR6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INPP5E(72 kDa inositol polyphosphate 5-phosphatase) converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns-P2.In mouse, highest protein expression was in brain, heart, and testis, with lower expression in thymus and lung, and very little expression in kidney, spleen, and liver(PMID:10764818). Defects in INPP5E are the cause of Joubert syndrome type 1 (JBTS1) and mental retardation-truncal obesity-retinal dystrophy-micropenis (MORMS)(PMID:19668215). It has 2 isoforms with the molecular mass of 66 kDa and 70 kDa.

实验方案

Product Specific Protocols
IF protocol for INPP5E antibody 17797-1-APDownload protocol
IHC protocol for INPP5E antibody 17797-1-APDownload protocol
IP protocol for INPP5E antibody 17797-1-APDownload protocol
WB protocol for INPP5E antibody 17797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Cell

Unraveling cellular complexity with transient adapters in highly multiplexed super-resolution imaging

Authors - Florian Schueder
View Article
humanWB,IF

Nat Commun

Identification of a component of the tubulin poly-glutamylase complex required for phosphoinositide homeostasis and cilium assembly and maintenance.

Authors - Binshad Badarudeen
View Article
humanIF

Nat Commun

Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis.

Authors - Qingwen Xu
View Article

Nat Commun

KIF13B establishes a CAV1-enriched microdomain at the ciliary transition zone to promote Sonic hedgehog signalling.

Authors - Kenneth B Schou
View Article
humanIF

J Clin Invest

Ciliopathy-related B9 protein complex regulates ciliary axonemal microtubule posttranslational modifications and initiation of ciliogenesis.

Authors - Ruida He
View Article
humanIF

Sci Adv

Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.

Authors - Chuan Chen
  • KO Validated
View Article
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