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验证数据展示

  • WB analysis of HeLa using 21723-1-AP

    WB analysis of HeLa using 21723-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21723-1-AP (INPP5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 21723-1-AP

    WB analysis using 21723-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21723-1-AP (INPP5A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 21723-1-AP

    WB analysis using 21723-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21723-1-AP (INPP5A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

INPP5A Polyclonal antibody

INPP5A Polyclonal Antibody for WB, ELISA
Cat No. 21723-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

43 kDa inositol polyphosphate 5-phosphatase, 5PTASE, EC:3.1.3.56, Inositol polyphosphate-5-phosphatase A, Type I inositol 1,4,5-trisphosphate 5-phosphatase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, COLO 320 cells, HeLa cells, HepG2 cells, human brain tissue, rat brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21723-1-AP targets INPP5A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 INPP5A fusion protein Ag16380 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 inositol polyphosphate-5-phosphatase, 40kDa
别名 43 kDa inositol polyphosphate 5-phosphatase, 5PTASE, EC:3.1.3.56, Inositol polyphosphate-5-phosphatase A, Type I inositol 1,4,5-trisphosphate 5-phosphatase
计算分子量 412 aa, 48 kDa
观测分子量 43-48 kDa
GenBank蛋白编号BC096280
基因名称 INPP5A
Gene ID (NCBI) 3632
RRIDAB_10734438
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14642
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INPP5A (Inositol Polyphosphate-5-Phosphatase A) is a membrane-bound type I inositol 1,4,5-trisphosphate 5-phosphatase that is involved in the mobilization of intracellular calcium ions and the regulation of cellular responses to stimuli. It terminates calcium signaling by hydrolyzing inositol 1,4,5-trisphosphate (InsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4). INPP5A plays important roles in various cellular processes, including cell proliferation, apoptosis, vesicular transport, and insulin signaling. Additionally, INPP5A is crucial for the survival of cerebellar Purkinje cells. In certain cancers, such as cutaneous squamous cell carcinoma (cSCC) and esophageal squamous cell carcinoma (ESCC), the expression levels of INPP5A are closely related to disease progression and prognosis.

实验方案

Product Specific Protocols
WB protocol for INPP5A antibody 21723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Dev Cell

Functional genetic screen identifies ITPR3/calcium/RELB axis as a driver of colorectal cancer metastatic liver colonization.

Authors - Ryan H Moy
  • KD Validated
View Article