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验证数据展示

  • WB analysis using 12665-1-AP

    WB analysis using 12665-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12665-1-AP (INPP4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 12665-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 12665-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12665-1-AP (INPP4A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with INPP4A antibody included
  • IHC staining of mouse colon using 12665-1-AP

    IHC staining of mouse colon using 12665-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12665-1-AP (INPP4A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with INPP4A antibody included
  • IHC staining of human placenta using 12665-1-AP

    IHC staining of human placenta using 12665-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12665-1-AP (INPP4A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with INPP4A antibody included
  • IHC staining of human stomach cancer using 12665-1-AP

    IHC staining of human stomach cancer using 12665-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12665-1-AP (INPP4A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with INPP4A antibody included
  • IF Staining of A549 using 12665-1-AP

    IF Staining of A549 using 12665-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using INPP4A antibody (12665-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

INPP4A Polyclonal antibody

INPP4A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12665-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

EC:3.1.3.66, Inositol polyphosphate 4-phosphatase type I, Inositol polyphosphate-4-phosphatase type I A, INP4A, INPP4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human stomach cancer tissue, mouse colon tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy INPP4A Ready-To-Use IHC Kit. INPP4A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12665-1-AP targets INPP4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag3346

Product name: Recombinant human INPP4A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-301 aa of BC028361

Sequence: MTAREHSPRHGARARAMQRASTIDVAADMLGLSLAGNIQDPDEPILEFSLACSELHTPSLDRKPNSFVAVSVTTPPQAFWTKHAQTEIIEGTNNPIFLSSIAFFQDSLINQMTQVKLSVYDVKDRSQGTMYLLGSGTFIVKDLLQDRHHRLHLTLRSAESDRVGNITVIGWQMEEKSDQRPPVTRSVDTVNGRMVLPVDESLTEALGIRSKYASLRKDTLLKSVFGGAICRMYRFPTTDGNHLRILEQMAESVLSLHVPRQFVKLLLEEDAARVCELEELGELSPCWESLRRQIVTQYQTI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 inositol polyphosphate-4-phosphatase, type I, 107kDa
别名 EC:3.1.3.66, Inositol polyphosphate 4-phosphatase type I, Inositol polyphosphate-4-phosphatase type I A, INP4A, INPP4
计算分子量 972 aa, 109 kDa
观测分子量100-110 kDa
GenBank蛋白编号BC028361
基因名称 INPP4A
Gene ID (NCBI) 3631
RRIDAB_3669147
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96PE3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Inositol polyphosphate 4 phosphatase (INPP4A) belongs to the group of phosphoinositide phosphatases controlling proliferation, apoptosis, and endosome function by hydrolyzing phosphatidylinositol 3,4-bisphosphate. INPP4A is highly expressed in the central nervous system, which is shown to play a key role in glutamate excitotoxicity and cell proliferation. (PMID: 36653678, PMID: 31938306)

实验方案

Product Specific Protocols
WB protocol for INPP4A antibody 12665-1-APDownload protocol
IHC protocol for INPP4A antibody 12665-1-APDownload protocol
IF protocol for INPP4A antibody 12665-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Genome Med

Collateral metabolic vulnerabilities unveiled by loss of isozyme diversity in breast cancer.

Authors - Rui Ding
View Article
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