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验证数据展示

  • WB analysis using 12665-1-AP

    WB analysis using 12665-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12665-1-AP (INPP4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 12665-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 12665-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12665-1-AP (INPP4A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 12665-1-AP

    IF Staining of A549 using 12665-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using INPP4A antibody (12665-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

INPP4A Polyclonal antibody

INPP4A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12665-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Type I inositol 3,4-bisphosphate 4-phosphatase, INPP4, INP4A, Inositol polyphosphate-4-phosphatase type I A, Inositol polyphosphate 4-phosphatase type I

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12665-1-AP targets INPP4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 INPP4A fusion protein Ag3346 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 inositol polyphosphate-4-phosphatase, type I, 107kDa
别名 Type I inositol 3,4-bisphosphate 4-phosphatase, INPP4, INP4A, Inositol polyphosphate-4-phosphatase type I A, Inositol polyphosphate 4-phosphatase type I
计算分子量 972 aa, 109 kDa
观测分子量100-110 kDa
GenBank蛋白编号BC028361
基因名称 INPP4A
Gene ID (NCBI) 3631
RRIDAB_3669147
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96PE3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Inositol polyphosphate 4 phosphatase (INPP4A) belongs to the group of phosphoinositide phosphatases controlling proliferation, apoptosis, and endosome function by hydrolyzing phosphatidylinositol 3,4-bisphosphate. INPP4A is highly expressed in the central nervous system, which is shown to play a key role in glutamate excitotoxicity and cell proliferation. (PMID: 36653678, PMID: 31938306)

实验方案

Product Specific Protocols
WB protocol for INPP4A antibody 12665-1-APDownload protocol
IHC protocol for INPP4A antibody 12665-1-APDownload protocol
IF protocol for INPP4A antibody 12665-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols