验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, HEK-293 cells |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy® INO80 Ready-To-Use IHC Kit. INO80 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18810-1-AP targets INO80 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | INO80 homolog (S. cerevisiae) |
| 别名 | Chromatin-remodeling ATPase INO80, DNA helicase-related INO80 complex homolog 1, DNA helicase-related protein INO80, EC:3.6.4.-, hINO80 |
| 计算分子量 | 1556 aa, 177 kDa |
| 观测分子量 | 178 kDa |
| GenBank蛋白编号 | BC146785 |
| 基因名称 | INO80 |
| Gene ID (NCBI) | 54617 |
| RRID | AB_10598463 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9ULG1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
INO80, also named as INOC1 and KIAA1259, belongs to the SNF2/RAD54 helicase family. The INO80 complex remodels chromatin by shifting nucleosomes. INO80 recruited by YY1 to YY1-activated INO80. In vitro, INO80 has double stranded DNA-dependent ATPase activity
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for INO80 antibody 18810-1-AP | Download protocol |
| WB protocol for INO80 antibody 18810-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Genet Mammalian SWI/SNF continuously restores local accessibility to chromatin.
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Cell Stem Cell INO80 Facilitates Pluripotency Gene Activation in Embryonic Stem Cell Self-Renewal, Reprogramming, and Blastocyst Development.
| ||
Mol Cell Nascent RNA antagonizes the interaction of a set of regulatory proteins with chromatin. | ||
Mol Cell Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals.
| ||
Sci Adv Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair | ||
Nat Commun Resolution of R-loops by INO80 promotes DNA replication and maintains cancer cell proliferation and viability.
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