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验证数据展示

  • WB analysis of HepG2 using 60352-1-Ig

    WB analysis of HepG2 using 60352-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 60352-1-Ig

    IHC staining of human lung cancer using 60352-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 60352-1-Ig

    IHC staining of human lung cancer using 60352-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Inhibin beta A-Specific Monoclonal antibody

Inhibin beta A-Specific Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60352-1-Ig

产品说明书

CloneNo. 1F8D8

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, IHC, ELISA

别名

INHBA, Inhibin Beta A, 1F8D8, Activin beta-A chain, EDF

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, pig liver tissue, human testis tissue, L02 cells, human placenta tissue
Positive IHC detected inhuman lung cancer tissue, human oesophagus cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60352-1-Ig targets Inhibin beta A-Specific in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, pig
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 inhibin, beta A
别名 INHBA, Inhibin Beta A, 1F8D8, Activin beta-A chain, EDF
计算分子量 47 kDa
观测分子量 47 kDa
GenBank蛋白编号NM_002192
基因名称 Inhibin beta A
Gene ID (NCBI) 3624
RRIDAB_2881461
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08476
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Inhibin βA(INHBA) is a ligand in the transforming growth factor β (TGF-β) superfamily. Inhibin βA forms a disulfide-linked homo-dimer known as activin A, which has been implicated in multiple biologic processes, including neoplastic progression. The inhibin βA can also bind the βB and α isoforms to form activin AB and inhibin, respectively.

实验方案

Product Specific Protocols
WB protocol for Inhibin beta A-Specific antibody 60352-1-IgDownload protocol
IHC protocol for Inhibin beta A-Specific antibody 60352-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols