验证数据展示
经过测试的应用
| Positive WB detected in | human kidney tissue, mouse heart tissue, mouse kidney tissue, mouse liver tissue |
| Positive IP detected in | mouse kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16187-1-AP targets ING3-specific in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | inhibitor of growth family, member 3 |
| 别名 | ING3, Eaf4, HSPC301, ING2, Inhibitor of growth protein 3 |
| 计算分子量 | 47 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | NM_019071 |
| 基因名称 | ING3 |
| Gene ID (NCBI) | 54556 |
| RRID | AB_2264993 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NXR8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Members of inhibitor of growth (ING) family function in inhibiting cell growth and inducing apoptosis. They are sequence homologous proteins. ING3 can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. This antibody is specifically against p47ING3.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for ING3-specific antibody 16187-1-AP | Download protocol |
| WB protocol for ING3-specific antibody 16187-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
