CoraLite® Plus 488-conjugated ING2 Monoclonal antibody
ING2 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human, rat, mouse
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2C3A7
验证数据展示
经过测试的应用
Positive FC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy ING2 Ready-To-Use IHC Kit. ING2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66512-1-Ig
推荐稀释比
Application | Dilution |
---|---|
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66512 targets ING2 in FC (Intra) applications and shows reactivity with Human, rat, mouse samples.
Tested Applications | FC (Intra) |
Tested Reactivity | Human, rat, mouse |
Immunogen | ING2 fusion protein Ag19291 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | inhibitor of growth family, member 2 |
Synonyms | ING1L, ING1Lp, ING2, Inhibitor of growth protein 2, p32, p33ING2 |
Calculated Molecular Weight | 280 aa, 33 kDa |
Observed Molecular Weight | 33-35 kDa |
GenBank Accession Number | BC030128 |
Gene Symbol | ING2 |
Gene ID (NCBI) | 3622 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9H160 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ING2, also named as ING1L, belongs to the ING family and contains a PHD-type zinc finger. As a candidate tumor suppressor, ING2 seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. It is a component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. Recent finding reported that ING2 regulates muscle differentiation by regulating chormatin remodeling program.
实验方案
Product Specific Protocols | |
---|---|
FC protocol for CL Plus 488 ING2 antibody CL488-66512 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |