验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, mouse liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17921-1-AP targets ING1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag12338 Product name: Recombinant human ING1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-279 aa of BC093942 Sequence: MLSPANGEQLHLVNYVEDYLDSIESLPFDLQRNVSLMREIDAKYQEILKELDECYERFSRETDGAQKRRMLHCVQRALIRSQELGDEKIQIVSQMVELVENRTRQVDSHVELFEAQQELGDTAGNSGKAGADRPKGEAAAQADKPNSKRSRRQRNNENRENASSNHDHDDGASGTPKEKKAKTSKKKKRSKAKAEREASPADLPIDPNEPTYCLCNQVSYGEMIGCDNDECPIEWFHFSCVGLNHKPKGKWYCPKCRGENEKTMDKALEKSKKERAYNR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | inhibitor of growth family, member 1 |
| 别名 | p24ING1c, p33, p33ING1, p33ING1b, p47 |
| 计算分子量 | 279aa,32 kDa; 422aa,47 kDa |
| 观测分子量 | 24 kDa, 33 kDa and 47 kDa |
| GenBank蛋白编号 | BC093942 |
| 基因名称 | ING1 |
| Gene ID (NCBI) | 3621 |
| RRID | AB_2878467 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UK53 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ING1 (Inhibitor of Growth 1) is a tumor suppressor gene located at human chromosome 13q33-34. It encodes multiple protein isoforms generated by alternative splicing and differential promoter usage, including p47ING1a (~47 kDa), p33ING1b (~33 kDa), p24ING1c (~24 kDa), and p27ING1d (~27 kDa) (PMID: 23412501). Among these, p33ING1b is the most abundantly expressed and best-characterized isoform in human cells (PMID: 41617915). All ING1 isoforms share a conserved C-terminal region containing a plant homeodomain (PHD) finger that recognizes the H3K4Me3 histone mark, enabling recruitment of histone deacetylase complexes to regulate chromatin structure and gene transcription. ING1 cooperates with p53 to induce cell cycle arrest and apoptosis, and its dysregulation is implicated in various cancers (PMID: 23255913).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ING1 antibody 17921-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
