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验证数据展示

  • WB analysis of A549 using 25561-1-AP

    WB analysis of A549 using 25561-1-AP

    WB result of IMUP antibody (25561-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMUP transfected A549 cells.

  • WB analysis using 25561-1-AP

    WB analysis using 25561-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25561-1-AP (IMUP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Featured Product
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IMUP Polyclonal antibody

IMUP Polyclonal Antibody for WB, ELISA
Cat No. 25561-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

C19orf33, H2RSP, HAI 2 related small protein, IMUP, IMUP 1, IMUP 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inEC109 cells, A549 cells, SGC-7901 cells, HeLa cells, L02 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25561-1-AP targets IMUP in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag22218

Product name: Recombinant human C19orf33 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-85 aa of BC060319

Sequence: MEFDLGAALEPTSQKPGVGAGHGGDPKLSPHKVQGRSEAGAGPGPKASNFRGLGKGRRLTAAPPSSKDTTALPTPAAAPAIRTRM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 19 open reading frame 33
别名 C19orf33, H2RSP, HAI 2 related small protein, IMUP, IMUP 1, IMUP 2
计算分子量 106 aa, 11 kDa
观测分子量20 kDa, 22 kDa
GenBank蛋白编号BC060319
基因名称 IMUP
Gene ID (NCBI) 64073
RRIDAB_3669485
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9GZP8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Immortalization up-regulated protein (IMUP), also known as C19orf33 or H2RSP, was first identified in SV40-immortalized fibroblasts and includes two isoforms, IMUP-1 and IMUP-2 (PMID: 14981917; 35142956). It is found primarily in the nucleus (PMID: 11606055). IMUP is reportedly upregulated in many cancer cells and tissues and its overexpression is associated with tumorigenicity and cell-cycle acceleration (PMID: 16962562; 35142956; 22886722). This polyclonal antibody raised against 1-106 aa of human IMUP can recognize both IMUP-1 and IMUP-2. The apparent molecular determined by SDS-PAGE might be due to post-translational modifications or anomalous migration behavior (PMID: 11080599).

实验方案

Product Specific Protocols
WB protocol for IMUP antibody 25561-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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