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IMP5 Polyclonal antibody

IMP5 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 12664-1-AP

Print datasheet

Synonyms

IMP 5, IMP5, intramembrane protease 5, SPP like 2C, SPPL2c

验证数据展示

  • WB analysis of HEK-293 using 12664-1-AP

    WB analysis of HEK-293 using 12664-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12664-1-AP (IMP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 12664-1-AP

    IP experiment of HEK-293 using 12664-1-AP

    IP result of anti-IMP5 (IP:12664-1-AP, 4ug; Detection:12664-1-AP 1:500) with HEK-293 cells lysate 3600ug.

  • IHC staining of human liver cancer using 12664-1-AP

    IHC staining of human liver cancer using 12664-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12664-1-AP (IMP5 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 12664-1-AP

    IHC staining of human liver cancer using 12664-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12664-1-AP (IMP5 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HEK-293 using 12664-1-AP

    IF Staining of HEK-293 using 12664-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 12664-1-AP (IMP5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, human liver tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12664-1-AP targets IMP5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen IMP5 fusion protein Ag3345 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name intramembrane protease 5
Synonyms IMP 5, IMP5, intramembrane protease 5, SPP like 2C, SPPL2c
Calculated Molecular Weight 684 aa, 74 kDa
Observed Molecular Weight 74 kDa
GenBank Accession NumberBC025401
Gene Symbol IMP5
Gene ID (NCBI) 162540
RRIDAB_2296163
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IUH8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IMP5 antibody 12664-1-APDownload protocol
IHC protocol for IMP5 antibody 12664-1-APDownload protocol
IF protocol for IMP5 antibody 12664-1-APDownload protocol
IP protocol for IMP5 antibody 12664-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

MIR-NATs repress MAPT translation and aid proteostasis in neurodegeneration.

Authors - Roberto Simone
View Article