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ILKAP Polyclonal antibody

ILKAP Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16017-1-AP

Print datasheet

Synonyms

ILKAP, PP2C DELTA

验证数据展示

  • WB analysis of human heart using 16017-1-AP

    WB analysis of human heart using 16017-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16017-1-AP (ILKAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 16017-1-AP

    IHC staining of human stomach cancer using 16017-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16017-1-AP (ILKAP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 16017-1-AP

    IHC staining of human stomach cancer using 16017-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16017-1-AP (ILKAP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman heart tissue, HepG2 cells, K-562 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16017-1-AP targets ILKAP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen ILKAP fusion protein Ag8826 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name integrin-linked kinase-associated serine/threonine phosphatase 2C
Synonyms ILKAP, PP2C DELTA
Calculated Molecular Weight 392 aa, 43 kDa
Observed Molecular Weight 43-45 kDa
GenBank Accession NumberBC006576
Gene Symbol ILKAP
Gene ID (NCBI) 80895
RRIDAB_2249188
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0C8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ILKAP antibody 16017-1-APDownload protocol
IHC protocol for ILKAP antibody 16017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols