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ILK Monoclonal antibody

ILK Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E10E4

Cat No : 67724-1-Ig

Print datasheet

Synonyms

DKFZp686F1765, ILK, ILK 1, ILK 2, ILK1, ILK2, integrin linked kinase, Integrin linked protein kinase, P59, p59ILK

验证数据展示

  • WB analysis using 67724-1-Ig

    WB analysis using 67724-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

经过测试的应用

Positive WB detected inU2OS cells, human placenta tissue, human peripheral blood platelets, rat colon tissue, mouse colon tissue, pig colon tissue, HepG2 cells, HeLa cells, HEK-293 cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67724-1-Ig targets ILK in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB, ELISA
Tested Reactivity Human, mouse, rat, pig
Immunogen ILK fusion protein Ag21289 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name integrin-linked kinase
Synonyms DKFZp686F1765, ILK, ILK 1, ILK 2, ILK1, ILK2, integrin linked kinase, Integrin linked protein kinase, P59, p59ILK
Calculated Molecular Weight 451 aa, 59 kDa
Observed Molecular Weight51 kDa
GenBank Accession NumberBC001554
Gene Symbol ILK
Gene ID (NCBI) 3611
RRIDAB_2882910
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13418
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ILK(Integrin-linked protein kinase) is also named as p59, PAT-4, DKFZp686F1765, ILK-1, ILK-2, ILK1, ILK2, p59ILK and belongs to the TKL Ser/Thr protein kinase family. This protein localizes to multiple sites, including the cytoplasm, and imports into the nucleus through sequences in its N-terminus, via active transport mechanisms that involve nuclear pore complexes(PMID: 18635968). ILK performs crucial roles in the control of intestinal cell and crypt-villus axis homeostasis-especially with regard to basement membrane fibronectin deposition-as well as cell proliferation, spreading, and migration(PMID:19885839).

实验方案

Product Specific Protocols
WB protocol for ILK antibody 67724-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols