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CD127/IL-7R Polyclonal antibody

CD127/IL-7R Polyclonal Antibody for FC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 17626-1-AP

Print datasheet

Synonyms

CD127, CDW127, IL 7 receptor subunit alpha, IL 7R alpha, IL 7R subunit alpha, IL 7RA, IL7R, IL-7R, IL7RA, ILRA, interleukin 7 receptor

验证数据展示

  • WB analysis of mouse thymus using 17626-1-AP

    WB analysis of mouse thymus using 17626-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17626-1-AP (CD127/IL7R antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 17626-1-AP

    IP experiment of K-562 using 17626-1-AP

    IP result of anti-CD127/IL-7R (IP:17626-1-AP, 4ug; Detection:17626-1-AP 1:1000) with K-562 cells lysate 1200ug.

  • FC experiment of K-562 using 17626-1-AP

    FC experiment of K-562 using 17626-1-AP

    1X10^6 K-562 cells were stained with 0.2ug CD127/IL7R antibody (17626-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

经过测试的应用

Positive WB detected inmouse thymus tissue, K-562 cells
Positive IP detected inK-562 cells
Positive FC detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3600
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

17626-1-AP targets CD127/IL-7R in WB, IP, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications FC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen CD127/IL-7R fusion protein Ag11844 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interleukin 7 receptor
Synonyms CD127, CDW127, IL 7 receptor subunit alpha, IL 7R alpha, IL 7R subunit alpha, IL 7RA, IL7R, IL-7R, IL7RA, ILRA, interleukin 7 receptor
Calculated Molecular Weight 459 aa, 52 kDa
Observed Molecular Weight 56-58 kDa
GenBank Accession NumberBC067537
Gene Symbol IL7R
Gene ID (NCBI) 3575
ENSEMBL Gene IDENSG00000168685
RRIDAB_2126105
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16871
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD127, also known as IL-7R subunit alpha (IL-7RA), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells. IL-7R is a heterodimer composed of CD127 and the common-γ chain receptor (CD132). IL-7R plays critical roles in lymphocyte development and homeostasis. CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP).

实验方案

Product Specific Protocols
WB protocol for CD127/IL-7R antibody 17626-1-APDownload protocol
IP protocol for CD127/IL-7R antibody 17626-1-APDownload protocol
FC protocol for CD127/IL-7R antibody 17626-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

J Mammary Gland Biol Neoplasia

TGFß1 Stimulates Lymphatic Endothelial Cells to Produce IL7 and IL15, Which Act as Chemotactic Factors for Breast Cancer Cells with Mesenchymal Properties

Authors - Nikolina Giotopoulou
View Article
humanWB

Mol Med Rep

TET2 is involved in DNA hydroxymethylation, cell proliferation and inflammatory response in keratinocytes.

Authors - Xinxin Liu
View Article