验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, Jurkat cells, mouse liver tissue |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
产品信息
23048-1-AP targets IL-4I1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
文献引用应用 | IF |
经测试反应性 | human, mouse |
文献引用反应性 | human |
免疫原 | IL-4I1 fusion protein Ag19136 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | interleukin 4 induced 1 |
别名 | IL4I1, IL4 induced protein 1, hIL4I1, hFIG1, FIG1 |
计算分子量 | 567 aa, 63 kDa |
观测分子量 | 70 kDa |
GenBank蛋白编号 | BC131625 |
基因名称 | IL-4I1 |
Gene ID (NCBI) | 259307 |
RRID | AB_3085705 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q96RQ9 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Interleukin-4 Induced Gene 1 (IL4I1) is a secreted L-phenylalanine oxidase mainly produced by inflammatory antigen-presenting cells-in particular, macrophages present in T helper type 1 granulomas and in various types of tumors. In the last decade, it has been shown that IL4I1 is involved in the fine control of B- and T-cell adaptive immune responses. Preclinical models have revealed its role in cancer immune evasion. Recent clinical data highlight IL4I1 as a new potential prognostic marker in human melanoma. As a secreted enzyme, IL4I1 may represent an easily targetable molecule for cancer immunotherapy. IL4I1 protein has an estimated molecular weight of 75 kDa. (PMID: 31330829, PMID: 21469114)
实验方案
Product Specific Protocols | |
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WB protocol for IL-4I1 antibody 23048-1-AP | Download protocol |
IF protocol for IL-4I1 antibody 23048-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |