验证数据展示
经过测试的应用
| Positive IHC detected in | human colon tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 2 publications below |
产品信息
60296-1-Ig targets IL-1F7/IL-37 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2441 Product name: Recombinant human IL-1F7/IL-37 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-218 aa of BC020637 Sequence: MSFVGENSGVKMGSEDWEKDEPQCCLEDPAVSPLEPGPSLPAMNFVHTSPKVKNLNPKKFSIHDQDHKVLVLDSGNLIAVPDKNYIRPEIFFALASSLSSASAEKGSPILLGVSKGEFCLYCDKDKGQSHPSLQLKKEKLMKLAAQKESARRPFIFYRAQVGSWNMLESAAHPGWFICTSCNCNEPVGVTDKFENRKHIEFSFQPVCKAEMSPSEVSD 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 1 family, member 7 (zeta) |
| 别名 | IL1F7, IL37, IL-37, 7F1A1, FIL1 zeta |
| 计算分子量 | 218 aa, 24 kDa |
| GenBank蛋白编号 | BC020637 |
| 基因名称 | IL-1F7/IL-37 |
| Gene ID (NCBI) | 27178 |
| RRID | AB_2881411 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9NZH6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for IL-1F7/IL-37 antibody 60296-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Mol Immunol Common genetic heterogeneity of human interleukin-37 leads to functional variance. | ||
Sci Rep Interleukin-37 expression and its potential role in oral leukoplakia and oral squamous cell carcinoma. | ||
Lab Invest IL-37bΔ1-45 suppresses the migration and invasion of endometrial cancer cells by targeting the Rac1/NF-κB/MMP2 signal pathway.
| ||
Cell Death Dis IL-37b alleviates endothelial cell apoptosis and inflammation in Kawasaki disease through IL-1R8 pathway. | ||
Sci Immunol Myeloid cell-derived proteases produce a proinflammatory form of IL-37 that signals via IL-36 receptor engagement |
