验证数据展示
经过测试的应用
| Positive WB detected in | Recombinant protein, Recombinant protein protein |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
17835-1-AP targets IL-3 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | pig |
| 免疫原 |
CatNo: Ag11839 Product name: Recombinant human IL-3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 17-152 aa of BC066273 Sequence: GLQAPMTQTTSLKTSWVNCSNMIDEIITHLKQPPLPLLDFNNLNGEDQDILMENNLRRPNLEAFNRAVKSLQNASAIESILKNLLPCLPLATAAPTRHPIHIKDGDWNEFRRKLTFYLKTLENAQAQQTTLSLAIF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 3 (colony-stimulating factor, multiple) |
| 别名 | Hematopoietic growth factor, IL 3, IL3, IL-3, Interleukin 3, Mast cell growth factor, MCGF, MULTI CSF, P cell stimulating factor |
| 计算分子量 | 152 aa, 17 kDa |
| 观测分子量 | 20 kDa |
| GenBank蛋白编号 | BC066273 |
| 基因名称 | IL-3 |
| Gene ID (NCBI) | 3562 |
| RRID | AB_10644127 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P08700 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Interleukin-3 (IL-3) is a multilineage hematopoietic growth factor that promotes the proliferation, differentiation and survival of early multilineage hematopoietic progenitors. In particular, this cytokine plays a key role in stimulating the proliferation and survival of myeloid precursors. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders (PMID: 24103757;2698876;2809196)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for IL-3 antibody 17835-1-AP | Download protocol |
| WB protocol for IL-3 antibody 17835-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
