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IL2RG Polyclonal antibody

IL2RG Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, WB, ELISA

Conjugate

Unconjugated

Cat No : 11409-1-AP

Print datasheet

Synonyms

CD132, IL 2 receptor subunit gamma, IL 2R subunit gamma, IL 2RG, IL2RG, IL-2RG, IMD4, p64, SCIDX, SCIDX1

验证数据展示

  • WB analysis using 11409-1-AP

    WB analysis using 11409-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11409-1-AP (IL2RG antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • FC experiment of Jurkat using 11409-1-AP

    FC experiment of Jurkat using 11409-1-AP

    1X10^6 Jurkat cells were stained with 0.2ug IL2RG antibody (11409-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, Raji cells
Positive FC detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11409-1-AP targets IL2RG in WB, FC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, WB, ELISA
Tested Reactivity human
Immunogen IL2RG fusion protein Ag1911 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interleukin 2 receptor, gamma (severe combined immunodeficiency)
Synonyms CD132, IL 2 receptor subunit gamma, IL 2R subunit gamma, IL 2RG, IL2RG, IL-2RG, IMD4, p64, SCIDX, SCIDX1
Calculated Molecular Weight 369 aa, 42 kDa
Observed Molecular Weight 64-70 kDa
GenBank Accession NumberBC014972
Gene Symbol IL2RG
Gene ID (NCBI) 3561
RRIDAB_2264645
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31785
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The interleukin-2 receptor (IL-2R) is a heterotrimeric receptor consisting of three subunits, alpha (IL2RA), beta (IL2RB), and gamma (IL2RG). IL2RG, also known as CD132, is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in the gene of IL2RG cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.

实验方案

Product Specific Protocols
WB protocol for IL2RG antibody 11409-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols