验证数据展示
经过测试的应用
Positive WB detected in | NK-92 cells |
Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
IF | See 1 publications below |
产品信息
13602-1-AP targets CD122/IL-2RB in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse, rat |
免疫原 | CD122/IL-2RB fusion protein Ag3929 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | interleukin 2 receptor, beta |
别名 | CD122, CD122/IL-2RB, IL 2 receptor subunit beta, IL 2R subunit beta, IL 2RB, IL2RB, IL-2RB, interleukin 2 receptor, beta, P70 75, p75 |
计算分子量 | 551 aa, 61 kDa |
观测分子量 | 70-85 kDa |
GenBank蛋白编号 | BC025691 |
基因名称 | IL2RB |
Gene ID (NCBI) | 3560 |
ENSEMBL Gene ID | ENSG00000100385 |
RRID | AB_10641033 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P14784 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. The beta subunit (IL2RB, also known as CD122 and p75) is involved in receptor mediated endocytosis and transduces the mitogenic signals of nterleukin 2.
实验方案
Product Specific Protocols | |
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WB protocol for CD122/IL-2RB antibody 13602-1-AP | Download protocol |
IHC protocol for CD122/IL-2RB antibody 13602-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Ocul Surf Conditional deletion of CD25 in the corneal epithelium reveals sex differences in barrier disruption | ||
J Dermatol Sci Exosomes containing miR-1469 regulate natural killer cells by targeting CD122 in non-segmental vitiligo | ||
ACS Omega Vanillic Acid Protects PC12 Cells from Corticosterone-Induced Neurotoxicity via Regulating Immune and Metabolic Dysregulation Based on Computational Metabolomics |