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  • Featured Product
  • KD/KO Validated

IL-20RB Monoclonal antibody

IL-20RB Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A2A3

Cat No : 60221-1-Ig

Print datasheet

Synonyms

DIRS1, FNDC6, IL 20 receptor subunit beta, IL 20R beta, IL 20R2, IL 20RB, IL20RB, IL-20RB, interleukin 20 receptor beta

验证数据展示

  • WB analysis using 60221-1-Ig

    WB analysis using 60221-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60221-1-Ig (IL-20RB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 60221-1-Ig

    FC experiment of HeLa using 60221-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human IL-20RB (60221-1-Ig, Clone:1A2A3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inA431 cells, MCF-10A cells, A375 cells, hTERT-RPE1 cells, HeLa cells
Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60221-1-Ig targets IL-20RB in WB, FC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen IL-20RB fusion protein Ag14368 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name interleukin 20 receptor beta
Synonyms DIRS1, FNDC6, IL 20 receptor subunit beta, IL 20R beta, IL 20R2, IL 20RB, IL20RB, IL-20RB, interleukin 20 receptor beta
Calculated Molecular Weight 311 aa, 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC033292
Gene Symbol IL20RB
Gene ID (NCBI) 53833
RRIDAB_11182930
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6UXL0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-20 receptor subunit beta (IL20RB) is a single-pass type I membrane protein of the type II cytokine receptor family. The interleukin-20-receptor I complex (IL-20-RI) is composed of two chains, IL20RA and IL20RB. IL-20-RI is a receptor for IL-19, IL-20 and IL-24. IL20RB can also form a heterodimer with IL22RA1. The IL22RA1/IL20RB dimer is a receptor for IL20 and IL24.

实验方案

Product Specific Protocols
WB protocol for IL-20RB antibody 60221-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Elife

A systematic CRISPR screen reveals an IL-20/IL20RA-mediated immune crosstalk to prevent the ovarian cancer metastasis.

Authors - Jia Li
  • KD Validated
View Article